6 x 5 LC-MS/MS Peptide Reference Mix

V7491_6x5-LC-MS-3aMS-Peptide-Reference-Mix--50ul_3

V7495_6x5-LC-MS-3aMS-Peptide-Ref--Mix--200pmol_3

Peptide Mix and Free Software to Monitor LC/MS Instrument Performance

Determine mass spec instrument sensitivity and dynamic range
No manual calculations, maximized instrument performance with free PReMiS™ Software

Catalog Number:

Size

50μl 200pmol

Catalog Number: V7491

Please Enquire

Catalog Number: V7495

Please Enquire

A Complete Solution for Monitoring LC and MS Instrument Quality Control and Performance

In combination with an accompanying software tool, the 6 × 5 LC-MS/MS Peptide Reference Mix provides a convenient way to assess LC column performance and MS instrument parameters, including sensitivity and dynamic range.

Consists of 30 peptides: 6 sets of 5 isotopologues of the same peptide sequence, differing only in the number of stable, heavy-labeled amino acids incorporated into the sequence.
Labels are uniform ¹³C and ¹⁵N atoms.
Isotopologues are chromatographically indistinguishable; however, since they differ in mass, they are clearly resolved by mass spectrometry.
The isotopologues of each peptide are present in a series of tenfold dilutions. This format allows assessment of instrument dynamic range and sensitivity from a single run.

The 6 × 5 LC-MS/MS Peptides are AAA-qualified for accurate monitoring, and are compatible with multiple applications and neat or complex mixtures.

Read an ACS Analytical Chemistry article by J. Beri et al. featuring use of the 6 x 5 Reagent: Reagent for Evaluating Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Performance in Bottom-Up Proteomic Experiments.

Workflow with PReMiS™ Software

The complimentary PReMiS™ Software streamlines routine analysis by:

Eliminating time-consuming manual calculations
Ensuring consistent instrument performance over time
Allowing you to compare instrument-to-instrument performance over time

This diagram shows a standard workflow using PReMiS™ Software to analyze data generated by the 6 × 5 LC-MS/MS Peptide Reference Mix.

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Compatible with Neat and Complex Mixtures

Use neat format for standard instrument monitoring
Spike peptide mix into complex samples (i.e., human cell extract) to monitor instrument performance with relevant experimental samples

The data here show identification of accurate retention times in neat and in complex samples. Retention times were determined when evaluating neat format (500fmol loaded) and when spiking (1pmol) into human K562 cell extract, using provided PReMiS™ Software.

LC-MS/MS Instrument Parameter and Feature Details

Parameter/Feature	Category	Calculated by Software	Details
Mass Accuracy	MS	Yes	—
Performance Over Time	Both	Yes	Parameters like LC retention time, peak height and mass accuracy are graphically displayed based on date and time.
Multi-Instrument Comparisons	Both	Yes	—
Retention Time	LC	Yes	Time required for peptide being separated to elute from the chromatography column.
Peak Width	LC	Yes	Peak width is an indication of column efficiency.
Base Peak Height	LC	Yes	This measure of the signal response can be correlated to the ionization efficiency of the peptide.
Graphical XIC Analysis	LC	Yes	A graphical XIC (eXtracted Ion Chromatogram) is displayed for all 30 ions for visual confirmation of the detectability of all masses. This is especially important for analysis of the peptide mixture spiked into a complex background.
Lowest Detectable Quantity	MS	Yes	Lowest abundance detected is reported for each peptide sequence.
Linear Fit Analysis (Linear Dynamic Range¹)	MS	Yes	*The software does not directly calculate the full dynamic range of the instrument. However, up to 5 logs of dynamic range can be determined directly from the Linear Fit Analysis.
LOD and LOQ²	MS	No	The AAA-qualified peptides allow the mix to confirm instrument LOD and LOQ, the basis for the amount loaded, which then can be used to confirm consistency of detection limits.

Protocols

Complete Protocol

6 x 5 LC-MS/MS Peptide Reference MixPDF (5057 KB) – English

Specifications

Catalog Number:

What's in the box?

Item	Part #	Size	Concentration
6 × 5 LC-MS/MS Peptide Reference Mix	V749A	1 × 50μl	0.5pmol/μl
Aliquot Tubes	V750A	1 × 6 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 10,247,711 and 11,692,983; European Pat. No. 2956768; and other patents pending.