Promega Corporation

ApoTox-Glo™ Triplex Assay

The ApoTox-Glo™ Triplex Assay combines three assay chemistries to easily assess viability, cytotoxicity and apoptosis events in the same cell-based assay well. First, viability and cytotoxicity are determined by measuring two differential protease biomarkers simultaneously with the addition of a single nonlytic reagent containing two peptide substrates. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). The substrate enters intact cells, where it is cleaved to generate a fluorescent signal proportional to the...

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ApoTox-Glo™ Triplex Assay

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ApoTox-Glo™ Triplex Assay

 Components

  • GF-AFC Substrate

    G608A1 x 10μl
  • bis-AAF-R110 Substrate

    G609A1 x 10μl
  • Assay Buffer

    G610A1 x 10ml
  • Caspase-Glo® 3/7 Buffer

    G810A1 x 10ml
  • Caspase-Glo® 3/7 Substrate

    G811A1 x 1 bottle
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10ml
- G6320 € 404,00 Add to cart

ApoTox-Glo™ Triplex Assay

Close Window

ApoTox-Glo™ Triplex Assay

 Components

  • GF-AFC Substrate

    G608B1 x 50μl
  • bis-AAF-R110 Substrate

    G609B1 x 50μl
  • Assay Buffer

    G610A2 x 10ml
  • Caspase-Glo® 3/7 Buffer

    G810A5 x 10ml
  • Caspase-Glo® 3/7 Substrate

    G811A5 x 1 bottle
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


5 × 10ml
- G6321 € 1.758,00 Add to cart


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Storage Conditions

Store all components at –20°C protected from light.

For product intended use please see Patents & Disclaimers tab.

Overview of the ApoTox-Glo Triplex Assay protocol. Figure 1. Overview of the ApoTox-Glo™ Triplex Assay protocol.
Necrosis results using the ApoTox-Glo Triplex Assay protocol.Figure 2. Necrosis results using the ApoTox-Glo™ Triplex Assay protocol.

Ionomycin treatment of Jurkat cells for 6 hours results in dose-dependent decrease in viability and increase in cytotoxicity with no caspase-3/7 activation, consistent with primary necrosis.

Apoptosis results using the ApoTox-Glo Triplex Assay protocol.Figure 3. Apoptosis results using the ApoTox-Glo™ Triplex Assay protocol.

ApoTox-Glo™ Triplex Assay with suspension Jurkat cells treated with staurosporine. Staurosporine treatment for 6 hours results in a dose-dependent decrease in viability and increase in cytotoxicity with an increase in caspase-3/7 activity consistent with apoptosis.

Cytostasis results using the ApoTox-Glo Triplex Assay protocol.Figure 4. Cytostasis results using the ApoTox-Glo™ Triplex Assay protocol.

Camptothecin treatment of Jurkat cells for 48 hours results in dose-dependent decrease in viability, no cytotoxicity and increase in caspase-3/7 activity, consistent with cell-cycle arrest and early-phase apoptosis.

Camptothecin treatment of Jurkat cells for 48 hours results in dose-dependent decrease in viability, no cytotoxicity and increase in caspase-3/7 activity, consistent with cell-cycle arrest and early-phase apoptosis.

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Use Restrictions

G6320, G6321 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G6320, G6321 U.S. Pat. Nos. 7,416,854, 7,553,632 and other patents pending.

G6320, G6321 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G6320, G6321 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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