DeadEnd™ Colorimetric TUNEL System

The DeadEnd™ Colorimetric TUNEL System is a modified TUNEL Assay designed to provide simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level. The DeadEnd™ Colorimetric TUNEL System measures nuclear DNA fragmentation, an important biochemical indicator of apoptosis. The system can be used to assay apoptotic cell death in both tissue sections and cultured cells. The DeadEnd™ Colorimetric TUNEL System end-labels the fragmented DNA of apoptotic cells using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the 3´...

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The DeadEnd™ Colorimetric TUNEL System is a modified TUNEL Assay designed to provide simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level. The DeadEnd™ Colorimetric TUNEL System measures nuclear DNA fragmentation, an important biochemical indicator of apoptosis. The system can be used to assay apoptotic cell death in both tissue sections and cultured cells. The DeadEnd™ Colorimetric TUNEL System end-labels the fragmented DNA of apoptotic cells using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the 3´-OH DNA ends using the enzyme Terminal Deoxynucleotidyl Transferase (TdT). Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and the stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei are stained dark brown.

Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.# M6101) can be used to generate the positive control and is available separately.

Features - Benefits

• Assay Cells or Tissue: Detect apoptosis in thick tissue sections or assess cell morphology.
• Simplify: Includes DAB substrate and H₂O₂ for color detection and plastic coverslips that simplify sample handling.
• Proven Applications: Vibratome® sections of neuronal tissue, Jurkat cells, HL-60 cells.

Applications

For more information, see the Protocols & Applications Guide.

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