P2161_pGEM--4Z-Vector--20ug_3

Standard Cloning Vector That can also Efficiently Synthesize RNA In vitro

  • Blue/white screening to easily identify recombinant clones
  • Versatile vector used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

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Catalog Number: P2161

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Overview
Protocols
Specifications
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The pGEM®-4Z Vector is a standard cloning vector that can also efficiently synthesize RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 for selecting recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.

The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0280VA-W

pGEM®-4Z Vector GenBank® Accession Number X65305 and vector sequence text file.

Specifications

Catalog Number:

Lieferumfang

Produkt Katalognummer Größe

pGEM®-4Z Vector

P216A 1 × 20μg

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

BB

See Protocol for storage recommendations.

Resources

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