Horseradish Peroxidase-Conjugated Antibodies

Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme. The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The products (unless otherwise noted) are supplied as 1mg/ml solutions. A starting working dilution of 1:2,500 is suggested for...

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Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme. The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The products (unless otherwise noted) are supplied as 1mg/ml solutions. A starting working dilution of 1:2,500 is suggested for most Western blot, dot blot and ELISA applications. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.

Rabbit Anti-Chicken IgY, HRP Conjugate is a secondary antibody developed in rabbits against chicken IgY. Anti-Chicken IgY, HRP Conjugate recognizes both the heavy and light chains of IgY and has been validated for use in Western blots, dot blots and ELISAs.

Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed in donkeys against goat IgG. Donkey Anti-Goat IgG, HRP Conjugate shows reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and mouse IgG. For Western blot applications with chromogenic detection use at a starting dilution of 1:10,000.

Features - Benefits

• Extensive Validation: Use with confidence, as supported by numerous publications.
• Ready-to-Use Formulation: No need to dissolve the antibody.
• Flexible Dispensing: We can readily accommodate large-scale custom orders. (Please inquire.)

Applications

• Western blotting.
• Enzyme-linked immunosorbent assay (ELISA).
• Dot blotting.

References

1. Hu, D. et al. (2012) Novel insight into KLF4 proteolytic regulation in estrogen receptor signaling and breast carcinogenesis. J. Biol. Chem. 287, 13584–97.
2. Hesse, E. et al. (2010) Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J. Cell Biol. 191, 1271–83.
3. Massaad, C.A., Washington, T.M., Pautler, R.G. and Klann, E. (2009) Overexpression of SOD-2 reduces hippocampal superoxide and prevents memory deficits in a mouse model of Alzheimer's disease. Proc. Natl. Acad. Sci. USA 106, 13576–81.
4. Berkowitz, O., Jost, R., Pollmann, S. and Masle, J. (2008) Characterization of TCTP, the translationally controlled tumor protein, from Arabidopsis thaliana. Plant Cell 20, 3430–47.
5. Robertson, J.B., Stowers, C.C., Boczko, E. and Johnson, C.H. (2008) Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast. Proc. Natl. Acad. Sci. USA 105, 17988–93.

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