Promega Corporation

Fluorometer Comparison Guide

  GloMax-Multi+ Microplate with Instinct Software GloMax-Multi Microplate GloMax-Multi Jr Single Tube QuantiFluor-ST Fluorometer QuantiFluor-P Fluorometer
  GloMax®-Multi+ Microplate
with Instinct® Software
GloMax®-Multi
Microplate
GloMax®-Multi Jr
Single Tube
QuantiFluor®-ST
Fluorometer
QuantiFluor®-P
Fluorometer
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Catalog No. E8032 with Shaking
E9032 with Heating and Shaking
E7031 E6070 Base Instrument
E6080 Instrument with Luminescence Installed
E6090 E6100 Instrument with Green/Blue Channels
E6105 Instrument with UV/Blue Channels
Product Type 6-384-well Microplate Multimode Reader 96-well Microplate Multimode Reader Single Tube Multimode Reader Single Tube Fluorometer Single Tube Fluorometer
Description Versatile Microplate Reader with Shaking, Instinct® Software, and Optional Heating.
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State-of-the-art optics, instrument design and data handling. Superior performance, affordable with flexible purchasing options.
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Multifunctional single tube instrument for utmost flexibility.
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Compact, easy to use, and low cost fluorometer with optional Minicell Adapter.
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Compact, easy to use, and low cost fluorometer.
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Available Detection Modes Luminescence:
E8041 Luminescence
Fluorescence:
E8051 Fluorescence
Absorbance:
E8061 Visible
E9061 UV Visible
Luminescence:
E7041 Luminescence
Fluorescence:
E7051 Fluorescence
Absorbance:
E7061 Visible
Luminescence:
E6098 Luminescence (or installed in instrument E6080)
Fluorescence:
E6071 Blue
E6072 UV
E6073 Green
E6074 Red
E6075 GFPUV
Absorbance:
E6076 Absorbance Module. Add 1 or more filter paddle(s):
E6077 560nm Filter
E6078 600nm Filter
E6079 750nm Filter
Fluorescence Fluorescence
Read Type Glow, Flash, Kinetic, Repeat Glow, Flash, Kinetic, Repeat Glow, Kinetic, Repeat Discrete or Continuous Discrete or Continuous
Sample Format 6, 12, 24, 48, 96, 384-well plates 96-well plates 10 x 10 mm cuvette, 100 - 200 µl minicell cuvette (optional), 0.5 mL PCR tube (100 µl minimum, optional, fluorescence) or 1.5 ml microcentrifuge tube (Luminescence) 10 x 10 mm plastic cuvette, 0.5 mL PCR tube (100µl minimum, with optional PCR tube adapter) or 50 - 250 µl minicell vial (with optional adapter) 10 x 10 mm plastic cuvette, 0.5 mL PCR tube (100µl minimum, with optional PCR tube adapter) or 75 - 250 µl minicell vial (with optional adapter)
Shaking Four speeds (150, 300, 500 rpm, Variable) linear and orbital modes Not Applicable Not Applicable Not Applicable Not Applicable
Temperature Control Optional: 2° C above ambient temperature to 45° C +/- 0.75° C Not Applicable Not Applicable Not Applicable Not Applicable
Dispense Optional:
E7071 Single Injector
E7081 Dual Injectors

Selectable between 25 - 200 µl in 5 µl increments for 6 to 96-well plate formats.
Not Available for Fluorescence Not Applicable Not Applicable Not Applicable
Fluorescence          
Light Source Wavelength-matched LED Wavelength-matched LED Wavelength-matched LED Wavelength-matched LED Wavelength-matched LED
Detector PIN-photodiode PIN-photodiode Photodiode Silicon photodiode Silicon photodiode
Read Position Top reading Top reading Not Applicable Not Applicable Not Applicable
Wavelength Selection Snap-in Fluorescence Optical Kits Snap-in Fluorescence Optical Kits Snap-in Fluorescence Modules 2 modes 2 modes
Wavelengths UV (Ex 365 nm: Em 410 - 460 nm)

Blue (Ex 490 nm: Em 510 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 720 nm)

AFC (Ex 405 nm: EM 495 - 505 nm)
UV (Ex 365 nm: Em 410 - 460 nm)

Blue (Ex 490 nm: Em 510 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 720 nm)

AFC (Ex 405 nm: EM 495 - 505 nm)
UV (Ex 365 nm: Em 410 - 450 nm)

Blue (Ex 460 nm: Em 515 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 725 nm)

UV-GFP (Ex: 365 nm, Em: 515 - 570 nm)
UV (Ex 365, Em 440 - 470 nm)

Blue (Ex 460 nm, Em 515 - 575 nm)
UV (Ex 365, Em 440 - 470 nm), Blue (Ex 460 nm, Em 515 - 575 nm)

or

Green (Ex 525 nm, Em >570 nm),Blue (Ex 460 nm, Em 515 - 575 nm)
Detection Limit 0.5 fmol/200 µl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA 0.5 fmol/200 µl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA 6 ppt fluorescein, 450 pg/ml DNA 1 ng/ml dsDNA, 1 ng/ml RNA, 10 ng/ml dsDNA using Hoechst Dye 33258, 100 ng/ml protein 1 ng/ml dsDNA, 1 ng/ml RNA, 10 ng/ml dsDNA using Hoechst Dye 33258, 100 ng/ml protein
Linear Dynamic Range 6 logs, assay dependent 6 logs, assay dependent 6 logs, assay dependent 4 logs, assay dependent 4 logs, assay dependent
Read Out Relative Fluorescence Units, Direct Concentration Relative Fluorescence Units Fluorescence Standard Units, Direct concentration Direct concentration Direct concentration
Calibration Multi-point calibration   1 - 5 point calibration Single-point calibration Single-point calibration
Discrete Sample Averaging Sample readings are averaged over 0.5 second to improve accuracy Sample readings are averaged over 0.5 second to improve accuracy Sample readings are averaged over 5 seconds to improve accuracy Sample readings are averaged over 5 seconds to improve accuracy Sample readings are averaged over 5 seconds to improve accuracy
Instrument          
User Interface Built-in PC, touch screen navigation and operation Built-in PC, touch screen navigation and operation Touch screen navigation and operation Menu-driven keypad and LCD display Menu-driven keypad and LCD display
Data Output PC compatible ASCII text file format exported to USB flash drive, or connect to PC (not included) through USB or Serial Port PC or Mac compatible .csv file format exported to USB flash drive or connect to PC (not included) through optional PC Connect Kit Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available ASCII format through 9-pin RS-232 serial cable at 9600 baud rate (optional). Optional thermal printer available. ASCII format through 9-pin RS-232 serial cable at 9600 baud rate (optional). Optional thermal printer available.
External PC Requirements Optional. Windows XP or Vista Optional. Windows XP or Vista Optional. Windows XP SP2 or higher Windows XP SP2 or higher Windows XP SP2 or higher
Computer Interface USB type B or RS-232 port USB type B or RS-232 port 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud RS-232 port RS-232 port
Power 100 - 240 VAC, 50 - 60 Hz 100 - 240 VAC, 50 - 60 Hz Instrument: 12V DC, 0.7A Max, AC Adapter: 100-240V AC, 50/60Hz, 0.3A Instrument: 9V DC, 0.3A, AC Adapter: 100-240V AC, 50/60 Hz, 0.25A 4 AAA batteries
Auto-Shutoff Touch screen hibernates after 15 minutes of inactivity Touch screen hibernates after 15 minutes of inactivity Touch screen hibernates after 15 minutes of inactivity Not Applicable After 3 minutes of inactivity
Alarms Not Applicable Not Applicable Not Applicable High blank Low battery and high blank
Information          
Application Notes View Application Notes View Application Notes View Application Notes View Application Notes View Application Notes
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