PowerPlex® ESX and ESI Systems

A Real Choice in STR Analysis

The PowerPlex® ESX and ESI Systems meet the ENFSI recommendation for DNA profile sharing across Europe. They are based on five-color technology and allow co-amplification and detection of D3S1358, D8S1179, D18S51, D21S11, FGA, TH01, vWA, D2S441, D10S1248, D22S1045, D1S1656, D12S391, D2S1338, D16S539, D19S433, SE33 and Amelogenin. These kits are available in multiple formats, including the option to detect SE33, to accommodate various requirements or preferences. Additionally, the kits have increased tolerance to common inhibitors and increased sensitivity to obtain full profiles from low-level DNA and are robust enough to genotype degraded DNA samples through the use of mini-STR loci.

The systems are compatible with the Applied Biosystems 3130, 3130xl and 3500 Genetic Analyzers and ABI PRISM® 3100, 3100-Avant and 310 Genetic Analyzers.

STR Analysis Using the PowerPlex® ESX and ESI Systems

• More kit configurations: Satisfy your specific needs for processing database and casework samples.
• More loci: Include all ENFSI-recommended loci, with or without SE33.
• More mini-STR loci: Obtain more complete profiles from degraded DNA.
• More robust buffer: Achieve better results with inhibited samples.
• More complete kit: Get a complete system that includes Taq DNA polymerase in a convenient master mix, a size standard and amplification-grade water.

Consistent, Reliable Results

A DNA sample (0.5ng) heterozygous at all loci was amplified in a 25µl reaction with the PowerPlex® ESX 17 System for 30 cycles using a GeneAmp® PCR System 9700 thermal cycler. A loading cocktail containing CC5 Internal Lane Standard 500 and formamide was mixed with 1µl of amplified sample, and amplified fragments were analyzed using an Applied Biosystems 3130 Genetic Analyzer and a 3kV, 5-second injection. Data were analyzed using GeneMapper® ID software, version 3.2.1, and PowerPlex® ESX panels and bins files. Panel A. Fluorescein-labeled loci: Amelogenin, D3S1358, TH01, D21S11 and D18S51. Panel B. JOE-labeled loci: D10S1248, D1S1656, D2S1338 and D16S539. Panel C. TMR-ET-labeled loci: D22S1045, vWA, D8S1179 and FGA. Panel D. CXR-ET-labeled loci: D2S441, D12S391, D19S433 and SE33. Panel E. 60bp to 500bp fragments of the CC5 Internal Lane Standard 500.

Exceptional Sensitivity

A DNA sample (60pg) heterozygous at all loci was amplified in a 25µl reaction with the PowerPlex® ESI 17 Pro System for 30 cycles using a GeneAmp® PCR System 9700 thermal cycler. A loading cocktail containing CC5 Internal Lane Standard 500 Pro and formamide was mixed with 1µl of amplified sample, and amplified fragments were detected using an Applied Biosystems 3130 Genetic Analyzer and a 3kV, 5-second injection.

Robust Buffer Offers Superior Resistance to Inhibitors

A DNA sample (500pg) heterozygous at all loci was amplified in the presence of 300µM hematin in a 25µl reaction with the PowerPlex® ESX 17 System for 30 cycles using a GeneAmp® PCR System 9700 thermal cycler. A loading cocktail containing CC5 Internal Lane Standard 500 and formamide was mixed with 1µl of amplified sample, and amplified fragments were detected using an Applied Biosystems 3130 Genetic Analyzer and a 3kV, 5-second injection. Data were analyzed using GeneMapper® ID software, version 3.2.1, and PowerPlex® ESX panels and bins files.