HaloTag® Technology for Cell Imaging

Multiple Applications for Protein Functional Analysis–More Versatile than GFP

With HaloTag® Technology you can perform multicolor live- and fixed-cell imaging experiments using a single genetic construct. The technology is based on the formation of a covalent bond between a protein fusion tag and synthetic ligands, and is designed to enable understanding of protein function in a cellular and biochemical environment.

Featured Cell Imaging Applications

 

Temporal/Spatial Separation of Proteins in Live Cells

Fluorophores attached to HaloTag® ligands with different abilities to cross the cell membrane are used to regionally label a protein of interest.

 

Multiplex Live-Cell Imaging

The HaloTag® protein is not intrinsically fluorescent (unlike GFP). Therefore, the choice of fluorescent labels, including secondary and tertiary fluorophores, can be made after creating the HaloTag® fusion protein. This gives you more flexibility in choice of fluorophores than GFP.

 

Label Proteins in Fixed Cells--Combine with ICC for two-color Imaging

HaloTag® ligands retain their fluorescent properties after fixation, enabling multiplexing immunocytochemistry experiments.

 

Cell-to-Gel Analysis of Proteins

The HaloTag® protein-ligand interaction is stable and highly specific. The fluorescently labeled HaloTag® protein can be boiled with SDS-PAGE sample buffer and resolved on a gel without loss of fluorescent signal.