Promega Corporation

PinPoint™ Xa Protein Purification System

The PinPoint™ Xa Protein Purification System is designed for the production and purification of fusion proteins that are biotinylated in vivo. The DNA coding for the protein of interest is cloned into a PinPoint™ Vector downstream of a sequence encoding a peptide that becomes biotinylated in vivo. Biotinylated fusion proteins are produced in E. coli and are affinity-purified using the SoftLink™ Soft Release Avidin Resin. This proprietary resin allows elution of the fusion protein under nondenaturing conditions. The PinPoint™ Vectors feature the encoded endoproteinase Factor Xa (pronounced "ten a") proteolytic site...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

PinPoint™ Xa Protein Purification System

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PinPoint™ Xa Protein Purification System

 Components

  • SoftLink™ Soft Release Avidin Resin

    V201C1 x 3ml
  • PinPoint™ Xa-1 Vector

    V203A1 x 10μg
  • PinPoint™ Xa Control Vector

    V204A1 x 5μg
  • PinPoint™ Xa-2 Vector

    V205A1 x 10μg
  • PinPoint™ Xa-3 Vector

    V206A1 x 10μg
  • PinPoint™ Purification Column

    V556A1 x 1 each
  • Biotin

    V557A1 x 1ml
  • Streptavidin Alkaline Phosphatase

    V559A1 x 20μl
1 system
- V2020 € 797,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

PinPoint™ Xa-1 Vector

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PinPoint™ Xa-1 Vector

 Components

  • PinPoint™ Xa-1 Vector

    V203A1 x 10μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10μg
- V2031 € 238,00 Add to cart

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GenBank®/EMBL Accession Number

PinPoint™ Xa-1, U47626; PinPoint™ Xa-2, U47627; PinPoint™ Xa-3, U47628.

Storage Conditions

Store the PinPoint™ Purification Column at room temperature. Store all remaining components at 4°C. The vectors may be stored at –20°C.

For product intended use please see Patents & Disclaimers tab.

PinPoint Xa-1 Vector.Figure 1. PinPoint™ Xa-1 Vector.

The PinPoint™ Xa-2 and Xa-3 Vector circle maps are identical to PinPoint™ Xa-1 Vector, except for one and two, respectively, additional adenosines at position 394.

Use Restrictions

V2020, V2031 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V2020 Exclusively licensed under U.S. Pat. No. 6,072,039.

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