NanoLuc® Luciferase Technology

You Also May Be Interested In

• Nano-Glo® Luciferase Assay System

• FuGENE® HD Transfection Reagent

• GloMax® 96 Microplate Luminometer

• pGL4 Luciferase Reporter Vectors

GenBank^®/EMBL Accession Number

N1001=pNL1.1[Nluc] Vector Accession Number JQ437370; pNL1.1[Nluc] Vector sequence text file.
N1011=pNL1.2[NlucP] Vector Accession Number JQ437371; pNL1.2[NlucP] Vector sequence text file.
N1021=pNL1.3[secNluc] Vector Accession Number JQ437372; pNL1.3[secNluc] Vector sequence text file.
N1031=pNL3.1[Nluc/minP] Vector Accession Number JQ437373; pNL3.1[Nluc/minP] Vector sequence text file.
N1041=pNL3.2[NlucP/minP] Vector Accession Number JQ513370; pNL3.2[NlucP/minP] Vector sequence text file.
N1051=pNL3.3[secNluc/minP] Vector Accession Number JQ513371; pNL3.3[secNluc/minP] Vector sequence text file.
N1061=pNL2.1[Nluc/Hygro] Vector Accession Number JQ513372; pNL2.1[Nluc/Hygro] Vector sequence text file.
N1071=pNL2.2[NlucP/Hygro] Vector Accession Number JQ513373; pNL2.2[NlucP/Hygro] Vector sequence text file.
N1081=pNL2.3[secNluc/Hygro] Vector Accession Number JQ513380; pNL2.3[secNluc/Hygro] Vector sequence text file.
N1091=pNL1.1.CMV[Nluc/CMV] Vector Accession Number JQ513379; pNL1.1.CMV[Nluc/CMV] Vector sequence text file.
N1101=pNL1.3.CMV[secNluc/CMV] Vector Accession Number JQ513378; pNL1.3.CMV[secNluc/CMV] Vector sequence text file.
N1111=pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Vector Accession Number JQ513377; pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Figures

Figure 1. NanoLuc® Technology performs similarly to firefly luciferase but gives higher light output.

Figure 2. Relative size for different luciferases.

Figure 4. The bioluminescent reaction catalyzed by NanoLuc® luciferase.

/~/media/images/resources/figures/10500-10599/10559ma_650px.jpg?la=en

Figure 5. A comparison of light output and response dynamics between NanoLuc® and firefly luciferase constructs.

Figure 6. Reporter gene assays using secNluc.

Figure 7. pNL1.1[Nluc] Vector.

Figure 8. pNL1.2[NlucP] Vector.

Figure 9. pNL1.3[secNluc] Vector.

Figure 10. pNL3.1[Nluc/minP] Vector.

Figure 11. pNL3.2[NlucP/minP] Vector.

Figure 12. pNL3.3[secNluc/minP] Vector.

Figure 13. pNL2.1[Nluc/Hygro] Vector.

Figure 14. pNL2.2[NlucP/Hygro] Vector.

Figure 15. pNL2.3[secNluc/Hygro] Vector.

Figure 16. pNL1.1.CMV[Nluc/CMV] Vector.

Figure 17. pNL1.3.CMV[secNluc/CMV] Vector.

Figure 18. pNL3.2.NF-kB-RE[NlucP/NF-kB-RE/Hygro] Vector.

Use Restrictions

N1001, N1011, N1021, N1031, N1041, N1051, N1061, N1071, N1081, N1091, N1101, N1111 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.