pGEM®-luc DNA

The pGEM®-luc Vector is a cassette vector designed to be a source of the luc gene encoding firefly luciferase, which is found in the pGL2 Vectors. The plasmid is not intended for the expression of luciferase in eukaryotic or prokaryotic cells.

The pGEM®-luc Vector was constructed by positioning the luciferase gene (luc) in the center of the multiple cloning region of the pGEM®-11Zf(–) Vector, providing a number of unique restriction sites at both ends of the gene. Sites that are surrounded by parentheses are not unique, as additional sites for each also exist in the luciferase gene. Note also that using HindIII...

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The pGEM®-luc Vector is a cassette vector designed to be a source of the luc gene encoding firefly luciferase, which is found in the pGL2 Vectors. The plasmid is not intended for the expression of luciferase in eukaryotic or prokaryotic cells.

The pGEM®-luc Vector was constructed by positioning the luciferase gene (luc) in the center of the multiple cloning region of the pGEM®-11Zf(–) Vector, providing a number of unique restriction sites at both ends of the gene. Sites that are surrounded by parentheses are not unique, as additional sites for each also exist in the luciferase gene. Note also that using HindIII or NsiI to clone the luciferase gene will include upstream ATG codons, which may reduce the efficiency of expression in eukaryotes. The luciferase cassette does not contain the prokaryotic Shine-Delgarno sequence for bacterial expression.

The pGEM®-luc Vector is supplied with a glycerol stock of bacterial strain JM109.

Features - Benefits

• Flexibility: Provides a luciferase cassette with several unique cloning sites at both ends for analysis of transcriptional activity, mRNA processing, protein structure/function, or labeling of cells and viruses.

References

1. Ow, D.W. et al. (1986) Science 234, 856.
2. de Wet, J.R. et al. (1987) Mol. Cell Biol. 7, 725–37.
3. Wood, K.V. (1990) Promega Notes 28, 1–2.

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