Since 1996, Promega has offered a rabbit polyclonal antibody (pAb) that recognizes the dually phosphorylated, active form of Extracellular signal-Regulated protein Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK). This Anti-ACTIVE™ MAPK pAb has been reformulated in a smaller size and for use at a lower dilution. Both the original and the new Anti-ACTIVE™ MAPK pAb are affinity-purified, rabbit polyclonal antibodies capable of recognizing the dually phosphorylated pTEpY motif in the recombinant enzyme and in the native enzyme found in activated cell extracts. Additionally, we now offer a new Anti-ERK 1/2 pAb (pan ERK 1/2 pAb) that binds to isoforms 1 and 2 of both active and inactive ERK/MAPK. These two new antibodies are described in the context of Western analysis.
Promega Notes 69, 9.
Bruce W. Jarvis and Rich Moravec