Terminal Deoxynucleotidyl Transferase, Recombinant Protocol

Literature # 9PIM187

This enzyme catalyzes the repetitive addition of mononucleotides to the terminal 3′-OH of a DNA initiator, accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co²⁺ (as CoCl₂) in the reaction buffer allows the tailing of 3′-ends with varying degrees of efficiency.