CytoTox-Fluor™ Cytotoxicity Assay Technical Bulletin

Literature # TB350

The CytoTox-Fluor™ Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations. The CytoTox-Fluor™ Assay measures a distinct protease activity associated with cytotoxicity. The assay uses a fluorogenic peptide substrate (bis-alanyl-alanyl phenylalanyl-rhodamine 110; bis-AAF-R110) to measure dead-cell protease activity, which has been released from cells that have lost membrane integrity. The bis-AAF-R110 Substrate cannot cross the intact membrane of live cells and therefore gives no signal from live cells.

The CytoTox-Fluor™ Assay accommodates downstream multiplexing with many Promega luminescent assays or spectrally distinct fluorescent assay methods, such as assays measuring caspase activation, reporter expression or orthogonal measures of viability.