HaloTag® Mammalian Pull-Down and Labeling Systems Technical Manual

Literature # TM342

The HaloTag® Mammalian Pull-Down System (Cat.# G6504) is used to isolate and identify intracellular protein complexes from mammalian cells. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin, allowing rapid and efficient capture of dilute protein complexes. These benefits, coupled with the low nonspecific protein binding features of the HaloLink™ Resin, improve the rate of successful complex capture and identification from mammalian cells. The HaloTag® Mammalian Pull-Down and Labeling System (Cat.# G6500) also includes the HaloTag® TMRDirect™ Ligand, which allows the study of cellular localization, trafficking and protein turnover using the same HaloTag® genetic construct.