Promega Corporation

An Integrated Approach to Studying Apoptosis: From Gene Expression to Cellular Events

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Abstract

We induced apoptosis in Jurkat cell cultures and measured the DEVDase (caspase-3/7) activity using the Apo-ONE™ Homogeneous Caspase-3/7 Assay (Cat.# G7790, G7791). Total RNA was isolated from apoptotic and control cell cultures using the SV Total RNA Isolation System (Cat.# Z3100). This RNA provided the template for cDNA synthesis and gene-specific amplification using the ImProm-II™ Reverse Transcription System (Cat.# A3800). Together, these reagents allow a "systems approach" for studying apoptosis—from gene expression to cellular events.

Cell Notes 2, 4–6.

Katharine Miller, Rich Moravec, and Terry Riss

Promega Corporation
Publication Date: 2001

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