Promega Corporation

in vitro Transcription on DNA Templates Immobilized to Streptavidin MagneSphere®...

in vitro Transcription on DNA Templates Immobilized to Streptavidin MagneSphere® Paramagnetic

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Abstract

The immobilization of molecules has successfully aided research in many areas, including purification of proteins and the study of protein-protein and protein-nucleic acid interactions. We have used Promega's Streptavidin MagneSphere® Paramagnetic Particles to immobilize DNA templates for use in the study of RNA polymerase II complex formation and transcription in vitro. The ability to rapidly isolate transcription complexes formed on the immobilized templates and the ease with which reaction conditions can be manipulated are particularly helpful for dissecting the complex interactions inherent in transcription.

Promega Notes 64, 21.

Mingyi Liu and David H. Price

Department of Biochemistry, University of Iowa, Iowa City, IA
Publication Date: 1997

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