Promega Corporation

A New System for Cloning and Expressing Protein-Coding Regions

Share

  • Share
  • Print
  • Email
  • Download PDF

Abstract

The Flexi® Vector Systems provide a rapid, efficient and high-fidelity method to transfer protein-coding regions between vectors with various expression or peptide tag options. Inserts are efficiently transferred between Flexi® Vectors following restriction enzyme digestion, maintaining insert orientation and reading frame. During the initial cloning step, a lethal gene in the Flexi® Vector cloning region allows positive selection for ligation of the insert. Antibiotic resistance genes simplify selection of the desired clone during subsequent transfer to other Flexi® Vectors.

Promega Notes 89, 11–15.

Michael Slater, Jim Hartnett, Natalie Betz, Jami English, Ethan Strauss, Becky Pferdehirt and Elaine Schenborn

Promega Corporation
Publication Date: 2005

Download Article (347 KB)

Prefer a different language?

Ihr ausgwähltes Land istGermany. Ihre ausgewählte Sprache istDeutsch. Bitte wählen Sie die Sprache aus:

Dies ist richtig, bitte weiter gehen zu »

Ich brauche weitere Hilfe

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.