Dave Smith1, Joby Jenkins1, Brad Larson, B.A.2, Tracy Worzella, M.S.2
1TTP LabTech Limited, Cambridge, UK, 2Promega Corporation, Madison, WI
Here we demonstrate the ability to miniaturize the CellTiter-Glo® and Caspase-Glo® 3/7 Assays for high-throughput cell-based screening in low-volume 384- and 1536-well formats. The TTP LabTech Mosquito® liquid handling system was used for delivery of cells and reagent. The linear range, %CV, and Z′-factor data obtained demonstrate how these scalable assays and instrumentation offer ideal tools for HTS users studying drug effects on cells during the drug discovery process.