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Notes: In this study, the expression of various adipokine genes linked to inflammation was examined during differentiation of pre-adipocytes to adipocytes in primary culture. The effect of TNFα on expression of these adipokines in differentiated human adipocytes was also investigated. qPCR was used to measure expression levels of the various adipokines, including NGF, during development and upon TNFα exposure. In addition, secretion of adipokines into the culture media was measured by ELISA, the NGF E_max^® ImmunoAssay System being used to determine NGF levels. (3502)

Notes: In this study, implantation of human bone marrow stem/progenitor cells (MSCs) into the hippocampus of immunodeficient mice was found to stimulate proliferation, migration and differentiation of endogenous neural stem cells. Results of immunohistochemical analyses were confirmed by ELISA. The NGF E_max^® Immunoassay System was used to determine NGF levels in isolated hippocampi at various intervals after implantation of the MSCs. (3500)

Notes: Patients with locally advanced squamous cervical carcinoma were monitored for chemotherapy-induced peripheral neurotoxicity (CIPN). Plasma drawn from the study participants was stored at –80°C prior to testing for nerve growth factor (NGF)levels using the NGF E_max^® ImmunoAssay System. The authors note that a highly significant correlation existed between a decrease in circulating levels of NGF and an increase in the severity of CIPN. (3338)

Notes: The authors examine the role of brain-derived neurotrophic factor (BDNF) in alcohol addition. RNA was isolated from primary rat hippocampal neurons cultured in the absence or presence of ethanol. The RNA was reverse transcribed using the Reverse Transcription System, and BDNF and GPDH RNAs were quantitated by fluorescent real-time PCR. BDNF and nerve growth factor (NGF) protein levels were monitored using the BNDF and NGF Emax^® ImmunoAssay Systems. (3441)

Notes: To study the role of bone morphogenetic protein (BMP) in the development of peripheral sensory neurons, transgenic mice were created that overexpressed either a BMP inhibitor, noggin, or a ligand, BMP4. To determine if the increased trigeminal innervation and neuron numbers seen in these mice were due to increased neurotrophin levels, the mystacial (whisker) pads of 3-day-old mice were tested for the presence of NGF, BDNF and NT-3 using each of the corresponding E_max^® ImmunoAssay Systems. The results indicated there were no significant changes in neurotrophin levels except for a decrease in NT-3 in the noggin transgenic mice. (3337)

Notes: The authors studied the effects of a chronic intermittent cold stress regime on sympathetic nerve activation and ovarian physiology. To analyze whether this effect on noradrenaline was preceded by activation of the neurotrophic factor system responsible for growth and survival of sympathetic neurons, they measured nerve growth factor in adult female Sprague-Dawley rats using the NGF Emax^® ImmunoAssay System. (2772)

Notes: After inducing myocardial infarction in feral dogs, transcardiac NGF levels were measured in the serum from 0.5 hours to 1 month later. This difference in NGF concentration between the coronary sinus and aorta was measured in triplicate samples using the NGF E_max^® ImmunoAssay System. (3336)

Notes: The BDNF Emax^® ImmunoAssay System and the NGF Emax^® ImmunoAssay System were used to assay the levels of neurotrophinin in male Long–Evans rat brains. After cocaine consumption, brain tissue was homogenized and the BDNF and NGF levels assayed. (2805)

Notes: Cerebellar granule neurons were isolated from 8-day-old Wistar rats and were glucose deprived for 1 hour in Earl’s balanced salt solution. This solution was collected and assayed for NGF using the NGF Emax^® ImmunoAssay System. (2809)

Notes: Human amniotic membrane, either intact or epithelially denuded, was homogenized and NGF content measured using the NGF Emax^® ImmunoAssay System. (2807)

Notes: Protein and mRNA levels of various neurotrophins and neurotrophin receptors were analyzed in transected cervical vagus nerve and nodose ganglion. Nerve growth factor and neurotrophin-3 protein levels were measured using Promega's NGF E_max® ImmunoAssay System and NT-3 E_max® ImmunoAssay System, respectively. (2336)

Notes: Transplantation of olfactory ensheathing cells into lesions in the central nervous system is able to stimulate the growth of axons and in some cases restore functional connections. To investigate the mechanism, the authors quantitated the production of growth factors and expression of corresponding receptors by rat olfactory ensheathing cells. Levels of brain-derived neurotrophic factor, glial-cell-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 were monitored using Promega's BDNF E_max® ImmunoAssay System, GDNF E_max® ImmunoAssay System, NGF E_max® ImmunoAssay System, and NT-3 E_max® ImmunoAssay System, respectively.  (2318)

Notes: Regional and temporal patterns of neurotrophin messenger RNA and protein levels were characterized in the developing rat hippocampus, neocortex and cerebellum on postnatal days 1, 7, 14, 21, and 92. Brain-derived neurotrophic factor nerve growth factor, and neurotropin-3 protein levels were determined using Promega's BDNF E_max® ImmunoAssay System, NGF E_max® ImmunoAssay System, and NT-3 E_max® ImmunoAssay System. (2319)

Notes: In Alzheimer's disease, cholinergic neurons are lost from the medial septum and nucleus basalis of Meynert. Nerve growth factor and glial cell line-derived neurotrophic factor were examined to determine if these growth factors can rescue the cholinergic neurons. GDNF and NGF levels in the sections of the basal nucleus of Meynert in rat was determined using Promega's GDNF E_max® ImmunoAssay System and NGF E_max® ImmunoAssay System, respectively. Glial cell line-derived neurotrophic factor was capable of partially restoring the number of cholinergic neurons. (2325)

Notes: The levels of NGF in culture medium of numerous human pancreatic cancer cell lines was determined using Promega's NGF E_max® ImmunoAssay System. NGF-induced pancreatic cancer cell growth seemed to be dependent on the expression levels and the balance of the TrkA and p75 receptors. (2334)

Notes: The regulation of brain-derived neurotrophic factor, nerve growth factor and other neurotrophin ligands and receptors by estrogen was characterized in young adult and reproductively senescent rat brains. BNDF, NT-4, and NGF protein levels were monitored using Promega's BDNF E_max® ImmunoAssay, NT-4 E_max® ImmunoAssay System, and NGF E_max® ImmunoAssay Systems, respectively . (2320)

Notes: Mixed mouse cortical cultures containing both neurons and astrocytes were treated with zinc to induce neuronal death. Nerve growth factor augmented this zinc-induced neuronal death. The p75^NTR receptor and the p75^NTR-associated death executor (NADE) also play roles in zinc-triggered neuronal death. NGF levels were determined using Promega's NGF E_max® ImmunoAssay System. (2333)

Notes: The amounts of neurotrophins NGF, NT-3 and NT-4 were examined in the bladders of Wistar rats at various times after birth up to adulthood. NGF ranged from 5ng per bladder (day 5) to10ng per bladder (day 15). NT-3 ranged from less than 1ng per bladder at postnatal day 30 to as high as about 18ng per bladder in adulthood. NT-4 ranged from about 1ng per bladder on day 30 to more than 40ng per bladder in adulthood. NGF, NT-3,  and BDNF protein levels were determined with the NGF E_max^® ImmunoAssay System, NT-3 E_max^® ImmunoAssay System, and BDNF levels E_max^® ImmunoAssay System, respectively. Extraction procedures are detailed. (0228)

Notes: The levels of BDNF, NT-3 and NGF were examined in the cerebrospinal fluid of newborn rats (80 per sample) from postnatal day 1-21. NGF levels were consistently higher than the other neurotrophins ranging from 136pg/ml (day 21) to 607pg/ml (day 17) depending upon the day tested. NT-3 was the next most abundant ranging from approximately 40pg/ml (day 19) to 144pg/ml (day 7). BDNF was the least abundant ranging from 19.2pg/ml (day 5) to as high as 65pg/ml (day 13). NGF was determined with the NGF E_max^® ImmunoAssay System (NGF ELISA), NT-3 levels were determined with the NT-3 E_max^® ImmunoAssay System (NT-3 ELISA), and BDNF levels were determined with the BDNF E_max^® ImmunoAssay System (BDNF ELISA). (0167)

Notes: Bovine thecal cells were grown for three days then subcultured for 24 hours. The culture media was then removed and NGF levels determined using the NGF Emax^® ImmunoAssay System. (2810)

Notes: Brain-derived neurotrophic factor nerve growth factor, neurotrophin-3 levels were quantitated in neonatal and early postnatal rat hippocampus, septum, cortex/striatum and cerebellum using Promega's BDNF E_max® ImmunoAssay System, NGF E_max® ImmunoAssay System, and NT-3 E_max® ImmunoAssay System, respectively. (2312)

Notes: Levels of nerve growth factor in untreated and IL-1beta hippocampal neuronal cultures were monitored during homeostasis, hypoxia and reoxygenation. Untreated cells exhibited a decrease in both the expression and release of NGF under mild hypoxic stress whereas IL-1beta treated cells exhibited an increase in both expression and release of NGF. NGF levels were quantitated using Promega's NGF E_max® ImmunoAssay System. (2332)

Notes: A rat model of human polycystic ovarian syndrome in which NGF-producing neural progenitor cells were grafted into ovaries of juvenile rats was used to study the effect of NGF levels in ovary tissue. NGF levels in conditional culture medium from immortalized embryonic day 16 rat hippocampus-derived neural progenitor cells and in whole rat ovaries was determined using Promega's NGF Emax® ImmunoAssay System. (2331)

Notes: Thymulin induced thermal and mechanical hyperalgesia resulted in an increase in NGF, TNF alpha and PGE(2) levels in rat liver. NGF levels were determined with Promega's NGF E_max® ImmunoAssay System. (2335)

Notes: The NGF Emax^® ImmunoAssay System (NGF ELISA) was used to quantitate NGF in the rat medial septium, vertical limb of the diagonal band, area CA1 of the hippocampus and dentate guyrus of the hippocampus. Some detail of the referenced extraction procedure is provided. (2222)