MagneSil® Total RNA mini-Isolation System
Fast, Automated RNA Isolation from Small Amounts of Cells, Tissue or Blood
- Liquid handler-compatible
- Concentrated RNA eluted in as little as 15μl
Catalog Number:
Size
Catalog Number: Z3351
The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well procedure takes about 30 minutes to complete using a liquid-handling workstation.
Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.
- Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
- Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration.
- DNase I treatment is included to remove genomic DNA contamination.
Protocols
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
|
RNA Lysis Buffer (RLA) |
Z305D | 1 × 100ml | View Product |
|
DNase Stop Solution |
Z312D | 1 × 26.5ml | |
|
Yellow Core Buffer |
Z317D | 1 × 25ml | |
|
MnCl2, 0.09M |
Z318D | 1 × 5ml | |
|
MagneSil® RNA Paramagnetic Particles |
Z336D | 1 × 20ml | |
|
DNase I (lyophilized) |
Z358A | 4 × 1 vial | View Product |
SDS
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Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
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