GoTaq® Legionella qPCR and Viability qPCR Kits
Legionella qPCR Kits with Optional Live/Dead Cell Differentiation
- Get same-day results for quicker response and intervention
- Selectively amplify DNA from live cells, if desired, to evaluate the effectiveness of disinfection and treatment processes
- Identify L. pneumophila or Legionella spp., L. pneumophila and L. pneumophila Serogroup 1 in a single reaction for efficiency
- Ships at ambient temperature
Catalog Number:
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Size
$ 800.00
Catalog Number: AM2201
$ 1,200.00
Catalog Number: AM2202
$ 1,350.00
Catalog Number: AM2205
$ 1,750.00
Catalog Number: AM2206
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Detect Live Legionella with Speed and Sensitivity for Early Detection and Prevention of Legionella Outbreaks
Reliable detection of Legionella pneumophila in water supplies is important to prevent occurrence of Legionnaires’ disease. Legionella testing is commonly performed using culture-based approaches, which often take 7–10 days. PCR-based detection is much faster, but it cannot differentiate between live and dead cells, which can possibly lead to false-positive results. The Legionella qPCR Kits combine the rapid protocols of PCR with the option to discriminate between live and dead cells for fast, sensitive and specific Legionella pneumophila detection. We also offer a multiplex kit that can detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1 in a single assay.
GoTaq® Legionella pneumophila qPCR Kit (Cat.# AM2201) ISO Conformance Statement
The automated nucleic acid purification and amplification workflows adhere to the protocol, experimental design and calculation methodologies outlined in ISO 12869:2019 for the detection and quantification of Legionella pneumophila via qPCR.
GoTaq® Legionella spp/pneumophila/SG1 qPCR Kit (Cat.# AM2202) ISO Conformance Statement
The automated nucleic acid purification and amplification workflows employed in this kit adhere to the protocol, experimental design and calculation methodologies outlined in ISO 12869:2019 for the detection and quantification of Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1 via qPCR.
Validated Performance for Reliable Legionella Detection
The GoTaq® Legionella qPCR Kits have undergone independent evaluation to validate their performance in detecting Legionella species in water samples. This third-party study, conducted in accordance with ISO 12869:2019 standards, assessed assay specificity and DNA recovery. Key findings demonstrate high specificity, robust assay efficiencies, and consistent DNA recovery.
For the detailed evaluation, including performance data and methodology, access the full report summary below.
Enhance Your Risk Assessment with the Legionella Viability qPCR Kit by Differentiating Live/Dead Cells
The kit includes a cell-impermeable intercalating reagent that selectively binds to DNA from cells with compromised membranes, such as those that are dead, dying or lysed. Once bound, the molecule irreversibly modifies DNA, making it unamplifiable in PCR reactions. This ensures that signal from the qPCR detection reaction is exclusively from viable cells and not dead cells, therefore eliminating false-positive results and unnecessary investigations or corrective actions.
Amplification of DNA from heat-treated bacterial samples containing 103 CFU/ml treated with (+VR) or without (–VR) viability reagent.
Faster Results, Faster Intervention
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Triplex to Detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1
Knowing whether infectious non-pneumophila Legionella species are present can help inform mitigation and response plans. You can conveniently and efficiently detect Legionella pneumophila and members of the Legionella genus in the same qPCR reaction with the Legionella spp./Legionella pneumophila/L. pneumophila Serogroup 1 Viability qPCR Kit.
Shipping at Ambient Temperature
Learn more about our quality testing in the white paper.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Legionella Quant Standard |
AM208A | 1 × 100μl | 4 × 10^6 copies/μl |
L. pneumophila IPC-Primer-Probe Mix (20X) |
AM225A | 1 × 220μl | |
Legionella qPCR Master Mix (2X) |
AM240A | 2 × 1.1ml | |
Nuclease-Free Water |
P119E-C | 1 × 13ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
Patent Pending.
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Legionella Quant Standard |
AM208A | 1 × 100μl | 4 × 10^6 copies/μl |
L. pneumophila/spp/SG1 IPC-Primer-Probe Mix (20X) |
AM226A | 1 × 220μl | |
Legionella qPCR Master Mix (2X) |
AM240A | 2 × 1.1ml | |
Nuclease-Free Water |
P119E-C | 1 × 13ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
Patent Pending.
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Viability PCR Reagent |
AM220A | 1 × 50μl | 30mM |
Viability PCR Neutralization Buffer (10X) |
AM300A | 2 × 5ml | |
Legionella Quant Standard |
AM208A | 1 × 100μl | 4 × 10^6 copies/μl |
L. pneumophila IPC-Primer-Probe Mix (20X) |
AM225A | 1 × 220μl | |
Legionella qPCR Master Mix (2X) |
AM240A | 2 × 1.1ml | |
Nuclease-Free Water |
P119E-C | 1 × 13ml |
Patent Pending.
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Viability PCR Reagent |
AM220A | 1 × 50μl | 30mM |
Viability PCR Neutralization Buffer (10X) |
AM300A | 2 × 5ml | |
Legionella Quant Standard |
AM208A | 1 × 100μl | 4 × 10^6 copies/μl |
L. pneumophila/spp/SG1 IPC-Primer-Probe Mix (20X) |
AM226A | 1 × 220μl | |
Legionella qPCR Master Mix (2X) |
AM240A | 2 × 1.1ml | |
Nuclease-Free Water |
P119E-C | 1 × 13ml |
Patent Pending.
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
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