Caspase-Glo® 3/7 Assay System
Caspase Activity Assay for Apoptosis Detection
- High sensitivity caspase assay—requires fewer cells and less enzyme
- Simple add-mix-measure protocol—no sample preparation needed
- Easily scalable to 96-, 384- and 1,536-well plate formats
- Integrates seamlessly with the MyGlo® Reagent Reader
Catalog Number:
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Size
Catalog Number: G8090
Catalog Number: G8091
Catalog Number: G8093
Catalog Number: G8092
Catalog Number: MG1010
Homogeneous Assay Measures Caspase-3/7 Activity
The Caspase-Glo® 3/7 Assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity.
The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference than fluorescent- or colorimetric-based assays. The caspase activity assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. The assay delivers excellent Z´-factor values in cell and purified enzyme models. Using the MyGlo® Reagent Reader in conjunction with the ProNect™ Data Platform, you can visualize caspase activity with ease.
Need to measure caspase activity in 3D cultures? See our new Caspase-Glo® 3/7 3D Assay!
Simple Bioluminescent Detection of Caspase Activity
Luminescence Is Linear Over a Broad Range of Cell Numbers
Jurkat cells were treated with anti-Fas mAb for 4.5 hours to induce apoptosis or were left untreated. Caspase-Glo® 3/7 Reagent was added directly to the cells in 96-well plates and incubated for 1 hour before recording luminescence. Each point represents the average of 4 wells. The "no cell" blank control value has been subtracted from each.
Measure Dose Response to Apoptosis Inducers
Jurkat cells were seeded at 10,000 cells/well and treated for 20 hours with two compounds. Caspase-Glo® 3/7 Reagent was added to the cells and luminescence measured on the MyGlo® Reagent Reader. The dose-response graph shows an apoptotic response to bortezomib with no apoptotic response to palbociclib.
Multiplex Caspase 3/7 with Cytotoxicity and Cell Viability Assays
Notes
Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8090 provides sufficient reagents to perform 25 caspase activity assays; Cat.# G8091, 100 assays; Cat.# G8092 and G8093, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8090 provides sufficient reagents to perform 100 caspase activity assays; Cat.# G8091, 400 assays; Cat.# G8092 and G8093, 4,000 assays.
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810C | 1 × 2.5ml |
|
Caspase-Glo® 3/7 Substrate |
G811C | 1 × 1 bottle |
SDS
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Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810A | 1 × 10ml |
|
Caspase-Glo® 3/7 Substrate |
G811A | 1 × 1 bottle |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810A | 10 × 10ml |
|
Caspase-Glo® 3/7 Substrate |
G811A | 10 × 1 bottle |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810B | 1 × 100ml |
|
Caspase-Glo® 3/7 Substrate |
G811B | 1 × 1 bottle |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Lieferumfang
| Produkt | Katalognummer | Größe | Available Separately |
|---|---|---|---|
MyGlo™ Reagent Reader
|
MG1000 | 1 × 1 each | |
CellTiter-Glo® 2.0 Reagent |
G9241 | 1 × 10ml | View Product |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Resources
Fachartikel
- Using a Real Time Kinetic Cytotoxicity Assay to Determine when to Detect Apoptosis by Caspase-3/7 Activation
- Elevated expression of miR-494-3p is associated with resistance to osimertinib in EGFR T790M-positive non-small cell lung cancer
- Enhanced extrinsic apoptosis of therapy-induced senescent cancer cells using a death receptor 5 (DR5) selective agonist
- Targeted zinc-finger repressors to the oncogenic HBZ gene inhibit adult T-cell leukemia (ATL) proliferation
- Metformin attenuates rotenone-induced oxidative stress and mitochondrial damage via the AKT/Nrf2 pathway
- Endogenous retroviruses mediate transcriptional rewiring in response to oncogenic signaling in colorectal cancer
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