Lumit® IL-2 (Human) Immunoassay

Quantify Cytokine Release Without Tedious Wash Steps

The Lumit® IL-2 Immunoassay quantitatively measures released IL-2 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

Note: The total number of assays per kit will vary based on final well volume.

For standard well volumes:

100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.

How the Lumit® IL-2 Immunoassay Works

Primary antibodies to IL-2 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-2, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-2 levels.

Simple One-Plate Protocol, No Wash Steps

Large Dynamic Range of Lumit® IL-2 Detection

The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.

Assay	IL-2 Human Immunoassay
Limit of Detection (LOD)	11.2pg/ml (3 SD above background)
Dynamic Range	28.2pg/ml – 25ng/ml
Minimal Detectable Dose (MDD)	7.2pg/ml (2 SD above background)
Assay Time	70 minutes
Sample Type	Cell culture supernatants

SD=Standard Deviation

Detect IL-2 Release as a Marker of T Cell Activation

Data showing IL-2 release as a marker of T cell activation — Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Lumit® IL-2 (Human) Immunoassay reagents were added directly to treated cell wells to detect IL-2 release from the T cells.

Lumit® IL-2 Immunoassay is Scalable for High-Throughput Applications

Example data from Lumit IL-2 assay in 96- and 384-well format

Human PBMCs were plated in 96-well plates (left) or 384-well plates (right) at 40,000 cells/well or 10,000 cells/well, respectively, and treated with a cell stimulation cocktail to induce IL-2 release. The Lumit® IL-2 Immunoassay reagents were added directly to the cell culture wells. The calculated EC₅₀ values are similar in the 96-well plate and 384-well plate formats.

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

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For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocols

Complete Protocol

Lumit™ IL-2 (Human) Immunoassay Technical ManualPDF (2098 KB) – English

What's in the box?

Item	Part #	Size	Concentration
Lumit® Detection Substrate B	VB405A	1 × 160μl
Lumit® Detection Buffer B	VB406A	1 × 3.2ml
Human IL-2 Standard	W122A	1 × 25μl	10μg/ml
Anti-hIL-2 mAb-SmBiT, 250X	W123A	1 × 60μl
Anti-hIL-2 mAb-LgBiT, 250X	W124A	1 × 60μl

SDS

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Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

What's in the box?

Item	Part #	Size	Concentration
Lumit® Detection Substrate B	VB405A	5 × 160μl
Lumit® Detection Buffer B	VB406A	5 × 3.2ml
Human IL-2 Standard	W122A	1 × 25μl	10μg/ml
Anti-hIL-2 mAb-SmBiT, 250X	W123A	5 × 60μl
Anti-hIL-2 mAb-LgBiT, 250X	W124A	5 × 60μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

What's in the box?

Item	Part #	Size	Concentration
Lumit® Detection Substrate B	VB405B	1 × 1.25ml
Lumit® Detection Buffer B	VB406B	1 × 25ml
Human IL-2 Standard	W122A	1 × 25μl	10μg/ml
Anti-hIL-2 mAb-SmBiT, 250X	W123B	1 × 600μl
Anti-hIL-2 mAb-LgBiT, 250X	W124B	1 × 600μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

What's in the box?

Item	Part #	Size	Concentration
Human IL-2 Standard	W122A	1 × 25μl	10μg/ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover

Posters

Homogeneous, Bioluminescent Cytokine Assay Applied to a Monocyte Activation Test for Pyrogen Detection

Other Resources

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Lumit® IL-2 (Human) Immunoassay

No-Wash Immunoassay for IL-2 Detection

Quantify Cytokine Release Without Tedious Wash Steps

How the Lumit® IL-2 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Large Dynamic Range of Lumit® IL-2 Detection

Detect IL-2 Release as a Marker of T Cell Activation

Lumit® IL-2 Immunoassay is Scalable for High-Throughput Applications

Explore Additional Cytokine Detection Assays

Protocols

Complete Protocol

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Resources

Articles

Posters

Other Resources

Similar Products

Lumit® IL-1β Human/Mouse Immunoassay

Lumit® HMGB1 Human/Mouse Immunoassay

Lumit® Immunoassay Labeling Kit and Detection Reagents

Lumit® IL-4 (Human) Immunoassay

Lumit® IL-6 (Human) Immunoassay

Frequently Used With

Caspase-Glo® 1 Inflammasome Assay

LDH-Glo™ Cytotoxicity Assay

T Cell Activation Bioassay (NFAT/IL-2)

GloMax® Discover System

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