Cell Signaling
Lumineszenzbasierte Assays in verschiedenen Formaten. Von in situ, Biosensor-Analysen bis in vitro und High Throughput-Screening.
Cell Signaling Produktgruppen
Glycobiology
Luminescent-based assays for measuring glycosyltransferase enzyme activity.
GPCR Signaling Assays
Einfach anzuwendende, biolumineszenz-basierte Kits to detect zum Nachweis von cAMP, GTPase und der PDE-Aktivität.
Kinase Target Engagement
Target engagement assays and kinase-luciferase fusion vectors to measure compound binding to select kinases in live cells.
Kinase-Biologie
Kinase-Aktivitätsassays, Substrate und Reagenzien. Sensitiv, einfach anzuwenden und auf Ihre Mengenbedürfnisse abstimmbar.
PARP/DDR Target Engagement Assays
Target engagement tracers and NanoLuc® fusion vectors for measuring cellular compound binding to PARPs and select targets in the DDR pathway.
Signaling-Pathways
Hier finden Sie AMP und UDP Nachweisreagenzien, Wachstumsfaktoren und Phosphatase-Assays.
RAS/RAF Pathway Assays
Easy-to-use, bioluminescence-based assays for probing the targets in the RAS-RAF-MAPK pathway.
Reporter Vectors and Cell Lines
Reporter gene vectors including firefly, NanoLuc® and Renilla luciferases, GFP and dual-reporter coincidence vectors. Also find cell lines stably expressing firefly luciferase.
Top Cell Signaling Produkte für Ihr Labor
ADP-Glo™ Kinase Assay
Universal, luminescent kinase activity assay.
V6930, V9101, V9102, V9103, V9104
NanoBRET® Target Engagement Kinase Assays
Directly measure kinase-ligand binding affinity, occupancy and residence time in live cells.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
cAMP-Glo™ Assay
45-minute, high-throughput assay for cAMP in cells.
V1501, V1502
An Introduction to Cell Signaling
Cell signaling, or signal transduction, is the process by which information from the cell surface is transmitted to the nucleus via a complex network of interwoven signaling cascades. These signaling events regulate cellular responses like proliferation, differentiation, secretion and apoptosis.
Signal transduction cascades are generally triggered by the binding of ligands, such as growth factors, cytokines, neurotransmitters, or hormones, to a receptor. These receptors transmit the stimulus to the interior of the cell, where the signal is amplified and directed through a signaling pathway.
The propagation of the primary signal involves a wide array of enzymes with specialized functions. In many cases, the signal is propagated by post-translational modification of proteins. Protein phosphorylation, one of the most common post-translational modifications, plays a dominant role in almost all signaling events. In general, phosphorylation either activates or inactivates a given protein to perform a certain function.
Protein kinases and phosphatases are responsible for determining the phosphorylation state of cellular proteins and, thus, whether a signal gets transduced within a cell. Changes in the level, subcellular localization and activity of kinases and phosphatases have consequences for normal cell function and maintenance of cellular homeostasis.
Assays to detect the activity of kinases and other signaling enzymes are fundamental tools for researchers studying a wide variety of signaling events. They are also used widely for the evaluation of drug:target interactions in drug discovery applications.
