Molekularbiologische Enzyme und Reagenzien
Klonierungsenzyme wie Ligasen, Polymerasen und PNK, DNA-modifizierende Enzyme, Klonierungsvektoren, kompetente Zellen und Mutagenese-Systeme.
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Molecular Biology Enzymes and Reagents Resources
Introduction to Molecular Biology Enzymes and Reagents
The most basic tools that enable the core technologies of molecular biology are enzymes and reagents.
Cloning enzymes include DNA ligases, which are typically used for ligation of DNA inserts into vectors, DNA polymerases for initiation of DNA synthesis, and RNA polymerases for initiation of transcription of RNA from a DNA template. DNA modifying enzymes allow manipulation of DNA for specific purposes. For example, nucleases are used to degrade DNA and/or RNA. Each nuclease has its specific purpose. S1 Nuclease degrades single-stranded DNA and single-stranded RNA, leaving behind blunt-ended double-stranded DNA or RNA.
