Ribonuclease H

Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3′-OH and 5′-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. This endonuclease can be used in applications such as microarrays.

Reference

1. Goodwin, E.C. and Rottman, F.M. (1992) Nucl. Acids Res. 20, 916.

Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 50% (v/v) glycerol, 0.2mg/ml BSA.

Source: Recombinant E. coli strain.

QC Tests: Activity, DNase, RNase, endonuclease, cDNA synthesis.

Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl₂, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).