Cut Site:
A▼CCGG T
T GGCC▲A
Usage Note: Ends generated with AgeI can be directly ligated to ends generated using AccIII, NgoMIV or XmaI. The AgeI recognition site is not regenerated in the ligation product.
Incubation Conditions: Buffer K. 37°C.
Source: Agrobacterium gelatinovorum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol, 0.5mg/ml BSA.