Cut Site:
GCCN NNN▼NGGC
CGGN▲NNN NCCG
Notes:
- BglI recognizes the defined internal six bases of the SfiI recognition site and also cuts at the same position.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
Incubation Conditions: Buffer D. 37°C.
Source: Bacillus globigii.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 10–25% | 25–50% | 75–100% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 29 | 20 | 0 | 2 | 1 | 3 |
Protocols
Complete Protocol
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe | Konzentration | Available Separately |
|---|---|---|---|---|
|
Buffer D 10X Buffer |
R004A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
BglI |
R607A | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
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