MULTI-CORE™ Buffer Pack

The MULTI-CORE™ Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE™ Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE™ Buffer.

See the table of Promega 1X Restriction Enzyme Buffer Composition below.

1X Restriction Enzyme Buffer Composition.

Buffer	pH (at 37°C)	Tris-HCl (mM)	MgCl₂ (mM)	NaCl (mM)	KCl (mM)	DTT (mM)
A	7.5	6	6	6	—	1
B	7.5	6	6	50	—	1
C	7.9	10	10	50	—	1
D	7.9	6	6	150	—	1
E	7.5	6	6	100	—	1
F	8.5	10	10	100	—	1
G	8.2	50	5	—	—	—
H	7.5	90	10	50	—	—
J	7.5	10	7	—	50	1
K	7.4	10	10	—	150	1
L	9.0	10	3	100	—	—

MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

Notes:

For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.

Lieferumfang

Produkt	Katalognummer	Größe
MULTI-CORE™ 10X Buffer	R999B	3 × 1ml

SDS

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Analysezertifikat

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

Store at –20°C.

Wird oft zusammen gekauft

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

MULTI-CORE™ Buffer Pack

Part Numbers: R9991

Convenient Aliquots of the Promega Universal Restriction Enzyme 10X Buffer

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Buffer	pH (at 37°C)	Tris-HCl (mM)	MgCl₂ (mM)	NaCl (mM)	KCl (mM)	DTT (mM)
A	7.5	6	6	6	—	1
B	7.5	6	6	50	—	1
C	7.9	10	10	50	—	1
D	7.9	6	6	150	—	1
E	7.5	6	6	100	—	1
F	8.5	10	10	100	—	1
G	8.2	50	5	—	—	—
H	7.5	90	10	50	—	—
J	7.5	10	7	—	50	1
K	7.4	10	10	—	150	1
L	9.0	10	3	100	—	—

Buffer	pH (at 37°C)	Tris-HCl (mM)	MgCl₂ (mM)	NaCl (mM)	KCl (mM)	DTT (mM)
A	7.5	6	6	6	—	1
B	7.5	6	6	50	—	1
C	7.9	10	10	50	—	1
D	7.9	6	6	150	—	1
E	7.5	6	6	100	—	1
F	8.5	10	10	100	—	1
G	8.2	50	5	—	—	—
H	7.5	90	10	50	—	—
J	7.5	10	7	—	50	1
K	7.4	10	10	—	150	1
L	9.0	10	3	100	—	—

MULTI-CORE™ Buffer Pack

Part Numbers: R9991

Convenient Aliquots of the Promega Universal Restriction Enzyme 10X Buffer

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

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Americas

Pacific Asia

Europe

Buffer	pH (at 37°C)	Tris-HCl (mM)	MgCl₂ (mM)	NaCl (mM)	KCl (mM)	DTT (mM)
A	7.5	6	6	6	—	1
B	7.5	6	6	50	—	1
C	7.9	10	10	50	—	1
D	7.9	6	6	150	—	1
E	7.5	6	6	100	—	1
F	8.5	10	10	100	—	1
G	8.2	50	5	—	—	—
H	7.5	90	10	50	—	—
J	7.5	10	7	—	50	1
K	7.4	10	10	—	150	1
L	9.0	10	3	100	—	—