NdeI

Cut Site:

CA^▼TA  TG
GT   AT_▲AC

Notes:

1. Star activity may be observed with glycerol concentrations >10%.
2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
3. Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Neisseria denitrificans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: