Cut Site:
GC▼GGCC GC
CG CCGG▲CG
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated.
Incubation Conditions: Buffer D. 37°C.
Source: Nocardia otitidis-caviarum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 0.1% Triton® X-100, 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.