PowerQuant® System
5-Color, 4-Target Probe-Based qPCR Assay
- Quantifies total amount of amplifiable autosomal and Y-chromosomal DNA in single assay
- Consistently detects 0.5pg/µl human DNA
- Detects PCR inhibitors and assesses DNA integrity of your sample
Catalog Number:
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Catalog Number: PQ5002
Catalog Number: PQ5008
Catalog Number: DS1221
Accurate and Sensitive DNA Quantification on the First Try
The PowerQuant® System is a 5-color, 4-target probe-based qPCR assay that quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. This system provides flexible options for 4-point to 7-point standard curve. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors.
- Trust your zero quantification results—zero means zero
- Obtain reliable quantification results with more consistent auto/Y ratios
- Maximize your success in STR assays with straightforward DNA quality assessment
- Shorten time to results with a faster cycling time of approximately 1 hour
- New software allows sample name import, data analysis and customized visualization
The PowerQuant® System is optimized for use on the following instruments:
- Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6)
- Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2)
- Applied Biosystems® QuantStudio™ 5 Real-Time PCR System (Design and Analysis Desktop Software, Versions 1.5 and 1.5.1)
Learn more about how the Casework Direct System and the PowerQuant® System can help improve processing efficiency for sexual assault evidence kits (SAEK).
Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
Use DNA Quantification Results to Reliably Predict STR Results
The PowerQuant® System can detect DNA at concentrations below which an informative profile can be obtained, even when the maximum sample volume is used in an STR amplification reaction.
Representative PowerPlex® Fusion data obtained with DNA template. Casework samples often contain DNA mixtures from multiple persons. The Y-chromosomal target in the PowerQuant® System enables quantification of human male DNA in the sample, helping you evaluate mixtures of male and female DNA. The PowerQuant® System also allows you to make better decisions when faced with a partial or failed STR amplification by differentiating between PCR inhibition and DNA degradation.
Reliably Detect PCR Inhibitors
Reliable detection of inhibitors that can affect DNA quantification and downstream STR assays is a key feature of the PowerQuant® System. The internal PCR control (IPC) has roughly the same level of sensitivity to inhibitors as currently available downstream STR assays, allowing you to determine if a sample is appropriate for amplification or if additional processing is required.
IPC Cq shift predicts downstream STR inhibition
In contrast to the sample with the lower humic acid concentration, the IPC Cq shifts for the samples with higher humic acid (HA) concentration (400ng/µl) indicate the presence of an inhibitor and concomitantly show a significant reduction in STR peak heights in the PowerPlex® Fusion System electropherograms. In this example, the [Auto]/[D] ratios are not affected at these humic acid concentrations. This illustrates that the IPC is more sensitive to inhibitors than the degradation target, enabling distinction between sample inhibition and degradation.
Assess Integrity of Your DNA Sample
The system amplifies a short autosomal and a long autosomal target. After amplification, the PowerQuant® Analysis Software calculates the ratio of DNA concentrations for the shorter and longer amplicons. The resulting degradation ratio helps you determine if a DNA sample is degraded, rather than inhibited, and decide which, if any, STR system is most appropriate for downstream analysis. The experiment below illustrates that the [Auto]/[D] ratio is reflective of sample integrity.
Comparison of [Auto]/[Deg] values obtained from PowerQuant® quantification of degraded DNA sample on the Applied Biosystems® 7500 and QuantStudio™ 5 Real-Time qPCR Systems. Samples were exposed to increasing amounts of UV radiation.
Want to Automate?
Save time and improve precision by automating your DNA quantification setup. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplifications setup.
Automate your DNA Quantification Setup
Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe | Available Separately |
|---|---|---|---|
PowerQuant® Male gDNA Standard |
DD302A | 1 × 150μl | View Product |
PowerQuant® 20X Primer/Probe/IPC Mix |
DK930A | 1 × 220μl | |
PowerQuant® 2X Master Mix |
DP527A | 2 × 1.1ml | |
|
Water, Amplification Grade |
DW099A | 2 × 1,250μl | View Product |
PowerQuant® Dilution Buffer |
Y101A | 2 × 1.5ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
Not For Medical Diagnostic Use.Lagerhinweise
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
Lieferumfang
| Produkt | Katalognummer | Größe | Available Separately |
|---|---|---|---|
PowerQuant® Male gDNA Standard |
DD302A | 3 × 150μl | View Product |
PowerQuant® 20X Primer/Probe/IPC Mix |
DK930A | 4 × 220μl | |
PowerQuant® 2X Master Mix |
DP527A | 8 × 1.1ml | |
|
Water, Amplification Grade |
DW099A | 5 × 1,250μl | View Product |
PowerQuant® Dilution Buffer |
Y101A | 8 × 1.5ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
Not For Medical Diagnostic Use.Lagerhinweise
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
PowerQuant® Calibration Standard, FAM |
DS106A | 1 × 60μl |
PowerQuant® Calibration Standard, CFG540 |
DS107A | 1 × 60μl |
PowerQuant® Calibration Standard, TMR |
DS108A | 1 × 60μl |
PowerQuant® Calibration Standard, Q670 |
DS109A | 1 × 60μl |
PowerQuant® Calibration Standard, CXR |
DS110A | 1 × 60μl |
PowerQuant® Calibration Buffer |
DS111A | 1 × 30ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
Not For Medical Diagnostic Use.Lagerhinweise
Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.
Resources
Fachartikel
Erwähnungen in der Fachliteratur
-
Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuant® system.
2016
Forensic Sci. Int. Genet.
-- - Alle Erwähnungen ansehen
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