ONE-Glo™ Luciferase Assay System

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo™ Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughput setting.

Assay Advantages

• Room temperature or 4°C storage options. The extended stability of the ONE-Glo™ Reagent makes it more convenient for everyday use.
• Improved Assay Precision. The ONE-Glo™ Reagent is less sensitive to mixing and dispensing conditions, enhancing reproducibility. Ideal for use in high-density (384- and 1536-well) microplates.
• Brighter, Longer-Lasting Signal. Optimized for batch and continuous-process handling, the extended bright light output allows high sensitivity, especially for extended incubations.
• Reduced Unwanted Effects from Sample Components. The ONE-Glo™ Assay is less sensitive to culture media, phenol red and luciferase inhibitors than other luciferase assays.