ViviRen™ Live Cell Substrate

Part Numbers: E6491, E6492, E6495

Sensitive Quantitation of Renilla Luminescence in Live Cells

  • Engineered coelenterazine for live-cell reporter gene, BRET and RNAi assays
  • Permits real-time measurement in multiwell plates
  • High signal-to-background ratios
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Ausgewählte Katalognummer: E6491

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ViviRen™ Live Cell Substrate
0.37mg
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Generate Kinetic Profiles for Reporter Assays, BRET and RNAi in Living Cells

ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity.

Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are each supplied as a lyophilized solid.

Assay Advantages

  • Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers.
  • Unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine.
  • Multiplex with CellTiter-Glo® and other lytic assays for improved accuracy and precision.
Luminescence reaches near-maximum levels within minutes of ViviRen Live Cell Substrate or coelenterazine addition.

Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition. CHO cells stably expressing Renilla luciferase were exposed to 20µM ViviRen™ Live Cell Substrate or coelenterazine approximately 1.5 minutes after continuous luminescent reading was initiated. For more details, see Figure 5 of TM064.

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ViviRen™ Live Cell Substrate

E649A 1 × 0.37mg

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

AA

Store Cat.# E6491 at –70°C. Store Cat.# E6492 and E6495 at –20°C.

Eigenschaften

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ViviRen™ Live Cell Substrate

E649B 1 × 3.7mg

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

GG

Store Cat.# E6491 at –70°C. Store Cat.# E6492 and E6495 at –20°C.

Eigenschaften

You are viewing: E6495 Konfiguration ändern

Lieferumfang

Produkt Katalognummer Größe

ViviRen™ Live Cell Substrate

E649C 1 × 37mg

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

GG

Store Cat.# E6491 at –70°C. Store Cat.# E6492 and E6495 at –20°C.

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