Trypsin/Lys-C Mix, Mass Spec Grade

Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a

Trypsin LysC overnight digest schematic 11788ME-W-b

Data comparing trypsin and Trypsin/Lys-C digests. — Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Reproducible IgG (Rituximab) digestion with Trypsin Lys-C mix. — Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a

Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b

Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Protokolle

Complete Protocol

Trypsin/Lys-C Mix, Mass Spec Grade Technical ManualPDF (696 KB) – English

Lieferumfang

Produkt	Katalognummer	Größe
Resuspension Buffer	V181A	1 × 500μl
Trypsin/Lys-C Mix, Mass Spec Grade	V507A	1 × 20μg

SDS

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Analysezertifikat

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

Store Trypsin/Lys-C Mix, Mass Spec Grade, at –30°C to –10°C.

Lieferumfang

Produkt	Katalognummer	Größe
Resuspension Buffer	V181A	1 × 500μl
Trypsin/Lys-C Mix, Mass Spec Grade	V507B	1 × 100μg

SDS

Search for SDS

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

Store Trypsin/Lys-C Mix, Mass Spec Grade, at –30°C to –10°C.

Lieferumfang

Produkt	Katalognummer	Größe
Resuspension Buffer	V181A	1 × 500μl
Trypsin/Lys-C Mix, Mass Spec Grade	V507A	5 × 20μg

SDS

Search for SDS

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

Store Trypsin/Lys-C Mix, Mass Spec Grade, at –30°C to –10°C.

Wissen + Tools

Fachartikel

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Arg-C, Sequencing Grade

Sequencing grade endopeptidase for use alone or in combination with other proteases for protein analysis by mass spectrometry.

V1881

Asp-N, Sequencing Grade

Endoproteinase hydrolyzes Peptide Bonds on the N-Terminal Side of Aspartic and Cysteic Acid Residues (Asp and Cys)

V1621

Chymotrypsin, Sequencing Grade

Serine protease that preferentially hydrolyzes at carboxyl side of amino acids Tyr, Phe and Trp

V1061, V1062

Trypsin/Lys-C Mix, Mass Spec Grade

Part Numbers: V5071, V5072, V5073

Mix of Trypsin Gold, Mass Spec Grade, and rLys-C, Mass Spec Grade, for Improved Protein Digestion

Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Improved Reproducibility using Trypsin/Lys-C

Tolerant To Trypsin-Inhibiting Contaminants

Protokolle

Complete Protocol

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Wissen + Tools

Fachartikel

Trypsin/Lys-C Mix, Mass Spec Grade

Part Numbers: V5071, V5072, V5073

Mix of Trypsin Gold, Mass Spec Grade, and rLys-C, Mass Spec Grade, for Improved Protein Digestion

Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Improved Reproducibility using Trypsin/Lys-C

Tolerant To Trypsin-Inhibiting Contaminants

Protokolle

Complete Protocol

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Wissen + Tools

Fachartikel

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