The Wizard® DNA Clean-Up System provides a simple and effective way to purify linear and circular DNA (200–50,000bp) from many molecular biology reactions:
- restriction enzymes, including heat-stable restriction enzymes
- phosphatases and kinases
- DNA polymerases, including Taq DNA polymerase
- exonucleases and endonucleases, including DNase I but not RNases
- mononucleotides
- salts
Using a quick batch-column procedure, the entire process can be completed in 15 minutes or less with no organic extractions or ethanol precipitations. DNA is eluted in water or TE buffer, ready for use. Multiple preps may be processed easily at one time with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231).
This product requires plungers (not provided) for use with the purification protocol without a vacuum manifold.