RNA Extraction
Reagenzien zur Extraktion von Gesamt-RNA und miRNA aus Blut, Zellen, Gewebe, Pflanzen und anderen Probentypen.
We offer RNA extraction products for all your throughput needs.
Explore Nucleic Acid Extraction Protocols by Sample Type
The Technical Manual supplied with each kit contains recommended protocols for specific sample types. We continue to test additional sample types and publish the results as short Application Notes. Search our Application Notes database for your specific sample type of interest to find protocols for manual, Maxwell or plate-based methods.
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RNA Extraction Basics
Obtaining high yields of pure, intact RNA is crucial for the success of many molecular biology applications. Isolating intact RNA requires four steps: 1) Disruption of cells or tissue; 2) Inactivation of endogenous ribonuclease (RNase) activity; 3) Denaturation of nucleoprotein complexes; and 4) Removal of contaminating DNA and proteins. The most important step is the immediate inactivation of endogenous RNases that are released from membrane-bound organelles when cells are disrupted.
RNA purification methods typically use silica membrane-based, resin-based and magnetic options for nucleic acid binding and incorporate DNase treatment to remove contaminating genomic DNA. Purified RNA is then eluted from the solid support.
RNA is notoriously susceptible to degradation and RNases are ubiquitous. Many commercially available RNA purification methods include specific chemicals to inactivate RNases present in cell or tissue lysates and may also include RNase inhibitors to safeguard against RNA degradation throughout the procedure.
