Z5420_PolyATract--System-1000-w-3a-Magnetic-Stnd_3 Z5400_PolyATtract--System-1000--w-3ao-Magnetic-_3

Isolates mRNA Directly from Crude Cell or Tissue Lysates

  • mRNA Purification is complete 45 minutes or less
  • Works with tissue amounts from 5mg–2g per isolation
  • No lengthy ethanol precipitation steps, phenol:chloroform extractions, and overnight ultracentrifugation
  • Magnetic separation stand (Cat.# Z5410) accommodates 1.5ml, 2ml, 15ml and 50ml tube sizes

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Catalog Number: Z5420

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Catalog Number: Z5400

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Overview
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The PolyATtract® System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolations. This system uses the MagneSphere® technology for the purification of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The increased yield of mRNA using this system allows the detection of low-copy-number mRNAs in relatively small amounts of material using Northern blot analysis. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease (RNase) protection assays, primer extension and Northern blots.

The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.

Specifications

Catalog Number:

Lieferumfang

Produkt Katalognummer Größe Available Separately

Nuclease-Free Water

P119C 1 × 25ml View Product

GTC Extraction Buffer

Z522A 1 × 20ml

Biotinylated Oligo(dT) Probe

Z526B 1 × 50μl View Product

Dilution Buffer

Z536A 1 × 40ml

mRNA User Tubes

Z537A 4 × 10 each

0.5X SSC Solution

Z538B 3 × 125ml

PolyATtract® System 1000 Magnetic Separation Stand

Z541A 1 × 1 each View Product

Streptavidin MagneSphere® Paramagnetic Particles

Z548D 1 × 25ml

Beta-Mercaptoethanol

Z559A 1 × 900μl

Analysezertifikat

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

CC

Lieferumfang

Produkt Katalognummer Größe Available Separately

Nuclease-Free Water

P119C 1 × 25ml View Product

GTC Extraction Buffer

Z522A 1 × 20ml

Biotinylated Oligo(dT) Probe

Z526B 1 × 50μl View Product

Dilution Buffer

Z536A 1 × 40ml

mRNA User Tubes

Z537A 4 × 10 each

0.5X SSC Solution

Z538B 3 × 125ml

Streptavidin MagneSphere® Paramagnetic Particles

Z548D 1 × 25ml

Beta-Mercaptoethanol

Z559A 1 × 900μl

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

CC

Resources

No related resources available