PI3K Lipid Kinases

Phosphatidylinositol (PI) and its phosphorylated derivates, collectively called phosphoinositides, are important second messengers that are critical as signaling molecules and for cellular membrane remodeling. These derivatives are generated by a family of kinases called phosphoinositide lipid kinases (PIKs). 

We offer lipid kinase enzymes, substrates and detection systems for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The purified human recombinant PI3K enzymes are components of the PI3K Class I Profiling Kit (Cat.# V1690) that are available separately. 

Sources

PI3K (p110α/p85α), 20μg: Complex of recombinant N-terminal FLAG-tagged full-length human p110α (GenBank® Accession Number U79143) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p110α[E545K]/p85α), 20μg: Complex of recombinant N-terminal FLAG-tagged full-length human p110α (GenBank® Accession Number U79143) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p110α[H1047R]/p85α), 20μg: Complex of recombinant N-terminal FLAG-tagged full-length human p110α (GenBank® Accession Number U79143) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p110β/p85α), 20μg: Complex of recombinant N-terminal FLAG-tagged full-length human p110β (GenBank® Accession Number NM_006219) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p120γ), 20μg: Recombinant N-terminal His-tagged full-length human p120γ (GenBank® Accession Number NM _02649) expressed in Sf9 insect cells.

PI3K (p110δ/p85α), 20μg: Complex of recombinant N-terminal GST-tagged full-length human p110δ (GenBank® Accession Number NM_005026) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.