ImProm-II™ Reverse Transcriptase

cDNA Synthesis for the Analysis of Rare or Long Messages

ImProm-II™ Reverse Transcriptase enables robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The ImProm-II™ Reverse Transcriptase can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA templates.

Advantages

Full-Length RT-PCR: Reverse transcribe RNA templates up to 8.9kb.
Microarray-Compatible: Incorporate regular and Cy®3- and Cy®5-modified nucleotides.
Scalable and Flexible: Reaction volumes of 1–20μl can be used in subsequent PCR, and the optimized buffer also allows for coupled RT-PCR.
Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl₂ Solution also is included.
Versatile: Use with your thermostable polymerase of choice for two-step RT-PCR.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

View ImProm-II™ RT Citations

Protokolle

Complete Protocol

ImProm-II™ Reverse Transcriptase Product InformationPDF (107 KB) – English

Lieferumfang

Produkt	Katalognummer	Größe	Konzentration	Available Separately
MgCl₂	A351B	1 × 750μl	25mM	View Product
ImProm-II™ 5X Reaction Buffer	M289B	1 × 100μl
ImProm-II™ Reverse Transcriptase	M314B	1 × 10μl

SDS

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Analysezertifikat

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Nutzungseinschränkung

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Lagerhinweise

Store at –20°C.

Lieferumfang

Produkt	Katalognummer	Größe	Konzentration	Available Separately
MgCl₂	A351H	1 × 1.2ml	25mM	View Product
ImProm-II™ 5X Reaction Buffer	M289A	1 × 600μl
ImProm-II™ Reverse Transcriptase	M314A	1 × 100μl

SDS

Search for SDS

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Lagerhinweise

Store at –20°C.

Lieferumfang

Produkt	Katalognummer	Größe	Konzentration	Available Separately
MgCl₂	A351H	3 × 1.2ml	25mM	View Product
ImProm-II™ 5X Reaction Buffer	M289C	2 × 1,250μl
ImProm-II™ Reverse Transcriptase	M314C	1 × 500μl

SDS

Search for SDS

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Lagerhinweise

Store at –20°C.

ImProm-II™ Reverse Transcriptase

Part Numbers: A3801, A3802, A3803

Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages

cDNA Synthesis for the Analysis of Rare or Long Messages

Protokolle

Complete Protocol

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Wissen + Tools

Fachartikel

Erwähnungen in der Fachliteratur

SV Total RNA Isolation System

RNasin® Ribonuclease Inhibitor

RNasin® Ribonuclease Inhibitor

MagneSil® Total RNA Mini-Isolation System

ImProm-II™ Reverse Transcriptase

Part Numbers: A3801, A3802, A3803

Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages

cDNA Synthesis for the Analysis of Rare or Long Messages

Protokolle

Complete Protocol

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Wissen + Tools

Fachartikel

Erwähnungen in der Fachliteratur

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RNasin® Ribonuclease Inhibitor

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