Rabbit Reticulocyte Lysate System, Nuclease Treated

Part Numbers: L4960

L4960_Rabbit Reticulocyte Lysate- Nuclease-Treated_3
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In Vitro Translation of mRNA and Characterization of mRNA Products

  • Optimized for translation and ready to use
  • Contains an energy-regenerating system, a mix of tRNAs, hemin, potassium chloride and magnesium acetate

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Ausgewählte Katalognummer: L4960

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Rabbit Reticulocyte Lysate System, Nuclease Treated
30 reactions
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Identify mRNAs, Characterize Protein Products 

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterize their protein products and investigate transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).

Learn More: See the Protein Expression chapter of the Protocols & Applications Guide for more information.

Choose Your Configuration: Learn more about custom options for this product at: www.promega.com/custom/

Applications
  • In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein
  • Characterization of mRNA products
  • Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction
References
  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
  2. Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.

Eigenschaften

Lieferumfang

Produkt Katalognummer Größe Konzentration Available Separately

Reticulocyte Lysate, Nuclease Treated

L416A 5 × 200μl

Amino Acid Mixture Minus Cysteine

L447C 1 × 50μl 1mM

Luciferase Control RNA

L456A 1 × 10μl View Product

Amino Acid Mixture Minus Leucine

L995A 1 × 50μl 1mM

Amino Acid Mixture Minus Methionine

L996A 1 × 50μl 1mM View Product

Analysezertifikat

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

AA

Store at –70°C or below. Do not freeze-thaw the lysate more than two times.

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