High Capacity Magne® Streptavidin Beads and Goat Anti-Human Biotinylated IgG

V7820_High-Capacity-Magne-Streptavidin-Beads_3 V7830_Goat-Anti-human-Biotinylated-IgG_3

Enrich Antibody from Sera or Plasma with Magnetic Streptavidin Beads

  • Characterize a variety of antibody concentrations with a small amount of beads
  • Suitable for use with robotics and high-throughput platforms
  • Goat Anti-Human Biotinylated IgG is available separately

Catalog Number:

Choose Magne Beads or Anti IgG Ab

Size

Catalog Number: V7820

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Catalog Number: V7830

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Overview
Protocols
Specifications
Resources
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Magnetic Affinity Beads for Antibody Capture from Complex Solutions

High Capacity Magne® Streptavidin Beads are magnetic affinity beads with high specificity and high capacity for binding biotinylated antibodies and proteins. The magnetic beads are composed of iron encapsulated by macroporous cellulose, resulting in low nonspecific binding and making them ideal for use with complex biological samples. The beads also have excellent magnetic properties for rapid and efficient capture using a variety of magnetic stands.

High Capacity Magne® Streptavidin Beads are well suited for pharmacokinetics studies of therapeutic antibodies during preclinical studies. For example, biotinylated anti-human IgG bound to the High Capacity Magne® Streptavidin Beads can be used for enrichment of Human IgG from serum or plasma samples of non-primate animals and analyzed using mass spectrometry. The high capacity of the beads enables enrichment of antibodies over a wide concentration range using small amount of beads. Enrichment can be automated for high throughput and scaled up to handle various sample volumes. Cat.# V7820 is provided as a 20% slurry.

Goat Anti-Human Biotinylated IgG is available separately and provided at a concentration of 0.5mg/ml in phosphate-buffered saline (pH 7.4) with 0.1% sodium azide.

Highly-Specific Binding

13666MC-W
High binding specificity results in fewer contaminants and thus more pure antibody from complex biological mixtures, such as plasma or sera. These beads are well suited for use in pharmacokinetic studies of therapeutic antibodies during preclinical trials.

High Capacity Beads

13729MC-W
Data comparing binding capacity of streptavidin beds from several vendors (50µl bead volumes).

Rapid Magnetic Response

13746MC-W
Total samples (250µl for washes and starting material and 100µl of elutate) were loaded on plate and read on GloMax® Discover with Red Excitation and 655 emission. Average blank replicate values for TBS and 0.1% TFA were approximately 10 RFU. n=4.

Specifications

Catalog Number:

Lieferumfang

Produkt Katalognummer Größe

High Capacity Magne® Streptavidin Beads

V782A 1 × 3ml

Analysezertifikat

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Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

CC

See Protocol for detailed storage recommendations.

Lieferumfang

Produkt Katalognummer Größe

Goat Anti-Human Biotinylated IgG Ab

V783A 1 × 4ml

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

CC

See Protocol for detailed storage recommendations.

Resources

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