HaloTag® Protein Purification System
Enhanced Expression and Solubility of Recombinant Proteins with HaloTag® Ligands
- Covalent, efficient and specific capture of your protein of interest
- Overcome limitations (i.e., poor capture of proteins expressed at low levels, protein loss during washing) associated with affinity tags
- Sample size is available (choose Cat.# G6270)
Catalog Number:
Size
Catalog Number: G6280
Catalog Number: G6270
Enhanced Expression and Solubility of Purified Protein with Novel Tag
HaloTag® Protein Purification System is designed to purify proteins fused to the HaloTag® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin).
The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as to monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual #TM250.
Use the HaloTag® Protein Purification System for:
- Purification of poorly expressed fusion proteins
- Improved yield, purity and specific activity of soluble, functional proteins compared to His-Tag, GST and MBP affinity tags
- Enhance target protein expression in prokaryotic, mammalian and cell-free systems
- Recover tag-free target protein using TEV Protease cleavage
- In-gel detection and quantification of protein expression levels with fluorescent HaloTag® Ligands
Schematic of protein purification with HaloTag® fusion tagging.
Use of HaloTag® protein improved yields compared to yields from other tags.
Protocols
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe | Available Separately |
|---|---|---|---|
TEV Protease for use with HisLink™ Resin |
V109B | 1 × 1.6ml | |
|
HisLink™ Protein Purification Resin |
V882C | 1 × 5ml | View Product |
|
HaloLink™ Resin |
G1915 | 1 × 25ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,112,552 and 7,354,750.
Lieferumfang
| Produkt | Katalognummer | Größe | Available Separately |
|---|---|---|---|
TEV Protease for use with HisLink™ Resin |
V109A | 1 × 200μl | |
|
HisLink™ Protein Purification Resin |
V882D | 1 × 0.5ml | View Product |
|
HaloLink™ Resin |
G1913 | 1 × 2.5ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,112,552 and 7,354,750.
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