Bio-Glo-NB™ Lytic Luciferase Assay Systems
Bright, Long-Lasting Luciferase Reagent for ADCP-MoA Target Cell Killing Assays
- Detection reagent designed for HiBiT target cell killing bioassays using primary macrophage
- Simple add-mix-read protocol for rapid assay results
- Quality controlled and tested for optimal reliability
Lytic Luciferase Assay Systems
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| JB1101 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 10ml | Preis anfragen | |||
| JB1102 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 100ml | Preis anfragen | |||
| JB1103 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 10 × 100ml | Preis anfragen |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Quantify Targeted Cell Killing with Confidence
The Bio-Glo-NB™ Luciferase Assay Systems provide a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays. Bio-Glo-NB™ Assay Reagents contain a cell-impermeable LgBiT that complements a HiBiT-tagged protein of interest in the extracellular media, resulting in a bright, luminescent NanoBiT® luciferase. Bio-Glo-NB™ Luciferase Assay Systems are functionally tested for performance in the Target Cell Killing Bioassays and intended for use with those products.
Created with BioRender.com
Bio-Glo-NB™ Lytic Luciferase System: Assay Principle
The Bio-Glo-NB™ Lytic Luciferase Assay Systems are functionally tested for performance using ADCP-qualified macrophage in the Target Cell Killing Bioassays and intended for use with those products. When HiBiT expressing target cells are paired with primary macrophage and a biologic molecule, such as Rituximab, the target cells undergo antibody-dependent cellular phagocytosis (ADCP), which leads to degradation of HiBiT and decreased luciferase activity when LgBiT and the substrate are added.
Created with BioRender.com
Simple Add-Mix-Read Protocol
Protocols
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 1 × 0.1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110A | 1 × 0.2ml |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111A | 1 × 10ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 1 × 1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110B | 2 × 1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111B | 1 × 100ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 10 × 1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110C | 5 × 4ml |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111B | 10 × 100ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Resources
Featured Resource
The small size and bright luminescence of NanoLuc® luciferase bring exquisite sensitivity to many applications. From NanoBRET® technology, a BRET-based method to study protein:protein and protein:small molecule interactions, to NanoLuc® Binary Technology (NanoBiT), NanoLuc is the cornerstone for studying genetic responses and protein dynamics in cells at physiologically relevant levels. The flexibility of NanoLuc® technologies provides you with the bioluminescent building blocks to understand biological interactions or perform compound screening in ways that have never been possible before.
Zusätzliches Informationsmaterial
Related Products
Ähnliche Produkte
CellTiter-Glo® Luminescent Cell Viability Assay
A homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells.
G7570, G7571, G7572, G7573
Caspase-Glo® 9 Assay Systems
A homogeneous luminescent assay that measures caspase-9 activity.
G8210, G8211, G8212
Bio-Glo-NB™ TCK Luciferase Assay Systems
A highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays.
JB1001, JB1002, JB1003
T Cell Activation Bioassay (TCRαβ-KO)
Measure the potency of transgenic TCR constructs to activate T cells without the constraints of endogenous TCR expression.
GA1172, GA1162, GA1182, GA1210, GA1220, GA1230
Wird oft zusammen gekauft
Thaw-and-Use Primary Effector Cells
Consistent and robust method for target cell killing.
CS3055C02, CS3055C03, CS3055B03, CS3055B06, CS3055A15, CS3055A18
H929 (HiBiT) TCK Bioassay
Simple, fast and sensitive technology to specifically measure target cell death.
JA1511, JA1515, M2412, GA6080, CS3055A28, CS3055A30, CS3055B08, CS3055B09, CS3055C15, CS3055C16
Ramos (HiBiT) TCK Bioassay
Simple, fast and sensitive technology to specifically measure target cell killing.
JA1411, JA1415, M2472, GA6070, CS3055A20, CS3055A22, CS3055B02, CS3055B05, CS3055C13, CS3055C14
Raji (HiBiT) TCK Bioassay
Simple, fast and sensitive technology to specifically measure target cell killing.
JA1211, JA1215, M2452, GA6050, CS3055A14, CS3055A17, CS3055B16, CS3055B17, JA1251, JA1255, JA1261, JA1265, JA1271, JA1275, CS3055C09, CS3055C10
