GoTaq® G2 Flexi DNA Polymerase Protocol
Instructions for Use of Product(s)
M7801, M7805, M7806, M7808
Literature # 9PIM780
GoTaq® G2 Flexi DNA polymerase contains GoTaq® G2 Flexi DNA Polymerase, 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer, and 25mM MgCl2. The enzyme is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5′→3′ exonuclease activity. The 5X Green GoTaq® Flexi Buffer contains two dyes (a blue dye and a yellow dye) that separate during electrophoresis to indicate migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR. The Flexi Buffers do not contain magnesium, allowing easy optimization of magnesium concentration in amplification reactions.
Revised 4/18.