4-1BB Bioassay
Instructions for Use of Product(s)
JA2351, JA2355, JA3351, JA3355, JA2251, JA2255
Literature # TM570
The 4-1BB Bioassay, is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate 4-1BB. The assay consists of a genetically engineered Jurkat T cell line that expresses human 4-1BB and a luciferase reporter driven by a response element that can respond to 4-1BB ligand/agonist antibody stimulation. The 4-1BB Effector Cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation.
This Technical Manual is relevant for Cat.# JA2351, JA2355, JA2251, JA2255; JA3351 and JA3355 (Korea).
Summary of Changes
The following changes were made to the 6/25 revision of this document:
1. Revised text about the label in Section 3.
2. Removed an expired patent statement and added a patent statement.
3. Updated Lumit to a registered trademark .
4. Made minor text edits.
Revised 6/25.