IL-2Rβγ Bioassay Technical Manual
Instructions for Use of Product(s)
JA5101, JA5105, JA6901, JA6905, JA7901, JA7905
Literature # TM728
The IL-2Rβγ Bioassay is a bioluminescent cell-based assay designed to measure human IL-2Rβγ receptor stimulation or inhibition in a thaw-and-use format. The IL-2Rβγ Bioassay consists of a genetically engineered cell line, IL-2Rβγ Bioassay Cells. When IL-2 or IL-15 bind to the medium affinity CD122/CD132 heterodimeric receptor complex (IL-2Rβγ), receptor-mediated pathway signaling induces luminescence that can be detected upon addition of Bio-Glo-NL™ Reagent and quantified with a luminometer. In the absence of cytokine, no signaling occurs downstream of the CD122/CD132 complex and a luminescent signal is not generated.
This Technical Manual is relevant for Cat.# JA5101 and JA5105; JA6901 and JA6905 (Korea); JA7901 and JA7905 (Taiwan).
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Removed an expired patent statement.
2. Updated text about the label in Section 3.
3. Updated the Lumit trademark.
Revised 5/25.