Intracellular TE Nano-Glo® Substrate-Inhibitor and Intracellular TE Nano-Glo® Vivazine-Inhibitor Technical Manual
Instructions for Use of Product(s)
N2160, N2161, N2162, N2200, N2201
Literature # TM610
The Intracellular TE Nano-Glo® Substrate/Inhibitor and Intracellular TE Nano-Glo® Vivazine™/Inhibitor are designed for use with NanoBRET™ Target Engagement (TE) Assays. These target engagement assays are based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells, and to measure compound binding at select target proteins within intact cells. Measurement of NanoBRET™ signals for target-NanoLuc® luciferase fusion proteins located inside living cells requires three key components: a NanoBRET™ tracer, a NanoLuc® substrate and an extracellular NanoLuc® inhibitor. NanoLuc® substrates are designed to produce adequate luminescence for BRET measurements over a specified range of time (i.e., short or long term). The extracellular NanoLuc® inhibitor is an impermeable molecule designed to quench any NanoLuc® signal derived from the extracellular environment. The combination of these three key components allows robust measurement of BRET signals originating from NanoLuc® target fusions that are located exclusively within live, intact cells.
Printed in USA 2/20