PowerPlex® ESX 17 Fast System for Use on the Spectrum Compact CE System Technical Manual

Instructions for Use of Product(s)
DC1710, DC1711

Literature # TMD059

The PowerPlex® ESX 17 Fast System is an STR multiplex system used for human identification applications including forensic analysis, relationship testing and research use. This system allows co-amplification and four-color fluorescent detection of seventeen loci (sixteen STR loci and Amelogenin), including D18S51, D21S11, TH01, D3S1358, Amelogenin, D16S539, D2S1338, D1S1656, D10S1248, FGA, D8S1179, vWA, D22S1045, SE33, D19S433, D12S391 and D2S441. The PowerPlex® ESX 17 Fast System amplifies the loci recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) as mini‑STRs (<125bp; D2S441, D10S1248 and D22S1045) or midi-STRs (125–185bp; D1S1656 and D12S391). All necessary materials are provided to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. This manual contains a protocol to separate amplified products and detect separated material on the Spectrum Compact CE System.

Summary of Changes
The following changes were made to the 9/21 revision of this document:

  1. Expanded character rules table in Section 5.3.
  2. Updated disclaimers.

Revised 9/21.